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Article

Sox9 is a β-catenin-regulated transcription factor that enhances the colony-forming activity of squamous cell carcinoma cells

  • Authors:
    • Xue Mei Li
    • Yong Jun Piao
    • Kyung‑Cheol Sohn
    • Jeong-Min Ha
    • Myung Im
    • Young‑Joon Seo
    • Kyu Uang Whang
    • Jeung‑Hoon Lee
    • Young Lee
    • Chang Deok Kim
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Department of Dermatology, School of Medicine, Chungnam National University, Daejeon 301‑747, Republic of Korea, Department of Dermatology, College of Medicine, Soonchunhyang University, Seoul 330‑721, Republic of Korea
  • Pages: 337-342
    |
    Published online on: May 4, 2016
       https://doi.org/10.3892/mmr.2016.5210
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Abstract

Squamous cell carcinoma (SCC) is a common skin cancer, of which the incidence is relatively high, ranking second among the non‑melanoma skin cancers. It is known that numerous intracellular signal regulators are involved in the pathogenesis of SCC. The Wnt/β-catenin signaling pathway serves an important role in cancer development. However, the downstream effectors of β‑catenin remain to be clearly elucidated yet. The present study investigated the functional importance of Wnt/β‑catenin signaling in cutaneous SCC. β‑catenin expression was reduced using recombinant adenovirus expressing specific microRNA (miR). Knockdown of β‑catenin resulted in a marked reduction of the colony-forming activity of the SCC cells, SCC12. In an attempt to identify the β‑catenin downstream genes, it was found that Sox9 was regulated by β‑catenin in SCC12 cells. Overexpression of a constitutively active form of β‑catenin led to the induction of Sox9, while knockdown of β‑catenin resulted in downregulation of Sox9. When the expression of Sox9 was reduced using specific miR, colony-forming activity of the SCC12 cells was significantly reduced. When Sox9 was overexpressed in cells where β‑catenin was knocked down, it partially restored the colony‑forming potential. Taken together, the present results suggested that Sox9 is a β-catenin downstream transcription factor and is positively involved in SCC development.
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Copy and paste a formatted citation
Spandidos Publications style
Li XM, Piao YJ, Sohn KC, Ha J, Im M, Seo YJ, Whang KU, Lee JH, Lee Y, Kim CD, Kim CD, et al: Sox9 is a β-catenin-regulated transcription factor that enhances the colony-forming activity of squamous cell carcinoma cells. Mol Med Rep 14: 337-342, 2016.
APA
Li, X.M., Piao, Y.J., Sohn, K., Ha, J., Im, M., Seo, Y. ... Kim, C.D. (2016). Sox9 is a β-catenin-regulated transcription factor that enhances the colony-forming activity of squamous cell carcinoma cells. Molecular Medicine Reports, 14, 337-342. https://doi.org/10.3892/mmr.2016.5210
MLA
Li, X. M., Piao, Y. J., Sohn, K., Ha, J., Im, M., Seo, Y., Whang, K. U., Lee, J., Lee, Y., Kim, C. D."Sox9 is a β-catenin-regulated transcription factor that enhances the colony-forming activity of squamous cell carcinoma cells". Molecular Medicine Reports 14.1 (2016): 337-342.
Chicago
Li, X. M., Piao, Y. J., Sohn, K., Ha, J., Im, M., Seo, Y., Whang, K. U., Lee, J., Lee, Y., Kim, C. D."Sox9 is a β-catenin-regulated transcription factor that enhances the colony-forming activity of squamous cell carcinoma cells". Molecular Medicine Reports 14, no. 1 (2016): 337-342. https://doi.org/10.3892/mmr.2016.5210
Copy and paste a formatted citation
x
Spandidos Publications style
Li XM, Piao YJ, Sohn KC, Ha J, Im M, Seo YJ, Whang KU, Lee JH, Lee Y, Kim CD, Kim CD, et al: Sox9 is a β-catenin-regulated transcription factor that enhances the colony-forming activity of squamous cell carcinoma cells. Mol Med Rep 14: 337-342, 2016.
APA
Li, X.M., Piao, Y.J., Sohn, K., Ha, J., Im, M., Seo, Y. ... Kim, C.D. (2016). Sox9 is a β-catenin-regulated transcription factor that enhances the colony-forming activity of squamous cell carcinoma cells. Molecular Medicine Reports, 14, 337-342. https://doi.org/10.3892/mmr.2016.5210
MLA
Li, X. M., Piao, Y. J., Sohn, K., Ha, J., Im, M., Seo, Y., Whang, K. U., Lee, J., Lee, Y., Kim, C. D."Sox9 is a β-catenin-regulated transcription factor that enhances the colony-forming activity of squamous cell carcinoma cells". Molecular Medicine Reports 14.1 (2016): 337-342.
Chicago
Li, X. M., Piao, Y. J., Sohn, K., Ha, J., Im, M., Seo, Y., Whang, K. U., Lee, J., Lee, Y., Kim, C. D."Sox9 is a β-catenin-regulated transcription factor that enhances the colony-forming activity of squamous cell carcinoma cells". Molecular Medicine Reports 14, no. 1 (2016): 337-342. https://doi.org/10.3892/mmr.2016.5210
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