Beclin 1 promotes apoptosis and decreases invasion by upregulating the expression of ECRG4 in A549 human lung adenocarcinoma cells

  • Authors:
    • Wenyu Wang
    • Hongkun Fan
    • Xiaoyan Li
    • Gang Wu
    • Weida Zhao
    • Guihong Zhang
    • Guoqiang Zhao
    • Linwei Li
  • View Affiliations

  • Published online on: May 6, 2016     https://doi.org/10.3892/mmr.2016.5219
  • Pages: 355-360
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Abstract

Although Beclin 1 has been demonstrated to exert an important role in cell autophagy during carcinogenesis, its biological function in lung cancer has yet to be fully elucidated. A previous study by our laboratory identified that knockdown of Beclin 1 promoted cell growth and inhibited apoptosis in the A549 lung cancer cell line. In the present study, a Beclin 1 lentiviral expression vector was constructed, and an A549 cell line was established with a steady expression of Beclin 1. Furthermore, the effect of Beclin 1 overexpression on cell invasion and apoptosis, changes in the activities of the apoptosis‑associated caspases‑3 and ‑9, and the overexpression of esophageal cancer‑related gene 4 (ECRG4) were examined. The results demonstrated that the overexpression of Beclin 1 in A549 cells reduced cell invasion by Matrigel invasion assay and promoted apoptosis by flow cytometric analysis (P<0.01) compared with Lenex‑packaged lentiviral particles and non‑transfected control groups. Furthermore, the overexpression of Beclin 1 in A549 cells increased the activities of caspases‑3 and ‑9 and the expression of ECRG4 (P<0.01) compared with Lenex‑packaged lentiviral particles and non‑transfected control groups. In conclusion, the overexpression of Beclin 1 promoted apoptosis and decreased invasion by upregulating the expression of ECRG4 in A549 lung adenocarcinoma cells. Therefore, the selection of Beclin l as a target for gene therapy represents a more effective method for the treatment of lung cancer.
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July-2016
Volume 14 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang W, Fan H, Li X, Wu G, Zhao W, Zhang G, Zhao G and Li L: Beclin 1 promotes apoptosis and decreases invasion by upregulating the expression of ECRG4 in A549 human lung adenocarcinoma cells . Mol Med Rep 14: 355-360, 2016.
APA
Wang, W., Fan, H., Li, X., Wu, G., Zhao, W., Zhang, G. ... Li, L. (2016). Beclin 1 promotes apoptosis and decreases invasion by upregulating the expression of ECRG4 in A549 human lung adenocarcinoma cells . Molecular Medicine Reports, 14, 355-360. https://doi.org/10.3892/mmr.2016.5219
MLA
Wang, W., Fan, H., Li, X., Wu, G., Zhao, W., Zhang, G., Zhao, G., Li, L."Beclin 1 promotes apoptosis and decreases invasion by upregulating the expression of ECRG4 in A549 human lung adenocarcinoma cells ". Molecular Medicine Reports 14.1 (2016): 355-360.
Chicago
Wang, W., Fan, H., Li, X., Wu, G., Zhao, W., Zhang, G., Zhao, G., Li, L."Beclin 1 promotes apoptosis and decreases invasion by upregulating the expression of ECRG4 in A549 human lung adenocarcinoma cells ". Molecular Medicine Reports 14, no. 1 (2016): 355-360. https://doi.org/10.3892/mmr.2016.5219