MicroRNA-34a inhibits cell proliferation and induces cell apoptosis of glioma cells via targeting of Bcl-2

Duan,Junwei; Zhou,Kejun; Tang,Xiaoping; Duan,Jie; Zhao,Long

doi:10.3892/mmr.2016.5255

MicroRNA-34a inhibits cell proliferation and induces cell apoptosis of glioma cells via targeting of Bcl-2

Authors:
- Junwei Duan
- Kejun Zhou
- Xiaoping Tang
- Jie Duan
- Long Zhao
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Affiliations: Department of Neurosurgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Department of Pediatric Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China
Published online on: May 12, 2016 https://doi.org/10.3892/mmr.2016.5255
Pages: 432-438

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Abstract

Glioblastoma is a highly malignant brain tumor, characterized by the poor prognosis and high recurrence rates. Despite therapeutic strategies including surgery, radiotherapy and chemotherapy, the median survival of patients is only 14.6 months. MicroRNAs (miRNAs) have been considered as a novel type of gene regulator. Previous studies have demonstrated that the expression of miRNA‑34a (miR‑34a) is significantly associated with the grade and prognosis of glioma. However, the exact function of miR‑34a on glioma progression and underlying mechanisms remain to be elucidated. The present study investigated the function of miR‑34a in U87 human glioma cells by exogenously transfecting cells with an miR‑34a mimic. Overexpression of miR‑34a inhibited proliferation, and induced apoptosis of U87 cells. The current study also demonstrated that B‑cell lymphoma 2 (Bcl‑2) was the target gene of miR‑34a, as demonstrated by luciferase assays. Furthermore, restoring the expression of Bcl‑2 was indicated to partially block the miR‑34a‑induced apoptosis. Thus, data from the present study identified miR‑34a as a tumor suppressor in glioma by, at least partially, targeting Bcl‑2. This may provide future novel diagnostic and therapeutic strategies for human glioma.

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