Tm7sf2 may participate in the healing of burn wounds

Lei,Na; Wang,Peng; Meng,Qiang

doi:10.3892/mmr.2016.5310

Tm7sf2 may participate in the healing of burn wounds

Authors:
- Na Lei
- Peng Wang
- Qiang Meng
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Affiliations: Department of Burn and Plastic Surgery, First Affiliated Hospital of Shihezi University Medical College, Shihezi, Xinjiang 832008, P.R. China, Deparment of Plastic and Aesthetic Center, Yantai Yuhuangding Hospital, Yantai Shandong 264000, P.R. China, Department of Intensive Care Unit, Jining First People's Hospital, Jining, Shandong 272011, P.R. China
Published online on: May 19, 2016 https://doi.org/10.3892/mmr.2016.5310
Pages: 1002-1006

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Abstract

The transmembrane 7 superfamily member 2 (Tm7sf2) gene been reported to be involved in the reduced cholesterol levels of patients with large burn areas. To investigate the importance of Tm7sf2 in the burn wound healing process, a total of 10 Sprague‑Dawley rats underwent electrical burns. Blood serum was collected for the culture of HaCaT human keratinocyte cells. Tm7sf2 small interfering RNAs (siRNAs) were prepared and transfected into the normal‑ and burn serum‑cultured HaCaT cells. Monocyte‑endothelial cellular adhesion ability and cell proliferation, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the levels of autophagy proteins Beclin1 and LC3‑II, which were detected by western blot assay and real time‑quantitative polymerase chain reaction, and it was identified that Tm7sf2 siRNAs were successfully transfected. Cell proliferation was reduced and monocyte-endothelial cell adhesion increased in the burn serum group compared with the normal serum group (P<0.05). Additionally, the expression levels of Beclin1 and LC3‑II were increased, and the addition of siTm7sf2 had a similar effect as the burn serum. Thus, Tm7sf2 may take part in the burn wound healing process by interacting with LC3‑II and Beclin1, and targeting Tm7sf2 may have therapeutic benefits.

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