Yifuning postpones ovarian aging through antioxidant mechanisms and suppression of the Rb/p53 signal transduction pathway

  • Authors:
    • Lei Liang
    • Xu‑Hui Zhang
    • Bo Ji
    • Hui Yao
    • Xiao‑Mei Ling
    • Zhi‑Jian Guo
    • Hong‑Zhu Deng
    • Xin‑Rong Wu
  • View Affiliations

  • Published online on: May 23, 2016     https://doi.org/10.3892/mmr.2016.5322
  • Pages: 888-896
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Abstract

Yifuning is a traditional Chinese medicine recipe that has been used for many years in China for its effects on treating climacteric syndrome in women. The present study aimed to demonstrate the effects and underlying molecular mechanism of Yifuning on the ovaries of aging rats. Selected aging rats were administered different doses of Yifuning (1.0 or 2.0 g/kg by lavage), and after 6 weeks the rats were sacrificed. The activit of indicators of oxidative stress in the serum were measured. The expression levels of 8-oxo-2'-deoxyguanosine (8-OHDG) and p53 in the ovaries were examined using immunohistochemistry. The expression levels of the corresponding genes and proteins were detected by reverse transcription‑quantitative polymerase chain reaction and western blotting analyses, respectively. The results indicated that Yifuning significantly prevented ovarian failure, as indicated by improvements in estrous cycling, reproductive organ weights and sex hormone serum levels. Yifuning significantly increased the levels of superoxide dismutase, glutathione peroxidase, catalase and reduced malondialdehyde and hydrogen peroxide levels. Yifuning reduced DNA damage in the ovaries by reducing the expression of 8‑OHDG and p53. Treatment with Yifuning significantly reduced the age‑induced p19, p53, p21 and Rb activity in the ovaries. The present study demonstrates that Yifuning prevents ovarian failure and the mechanism involved is partly associated with antioxidants and suppression of the Rb/p53 signal transduction pathway.
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July-2016
Volume 14 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liang L, Zhang XH, Ji B, Yao H, Ling XM, Guo ZJ, Deng HZ and Wu XR: Yifuning postpones ovarian aging through antioxidant mechanisms and suppression of the Rb/p53 signal transduction pathway. Mol Med Rep 14: 888-896, 2016.
APA
Liang, L., Zhang, X., Ji, B., Yao, H., Ling, X., Guo, Z. ... Wu, X. (2016). Yifuning postpones ovarian aging through antioxidant mechanisms and suppression of the Rb/p53 signal transduction pathway. Molecular Medicine Reports, 14, 888-896. https://doi.org/10.3892/mmr.2016.5322
MLA
Liang, L., Zhang, X., Ji, B., Yao, H., Ling, X., Guo, Z., Deng, H., Wu, X."Yifuning postpones ovarian aging through antioxidant mechanisms and suppression of the Rb/p53 signal transduction pathway". Molecular Medicine Reports 14.1 (2016): 888-896.
Chicago
Liang, L., Zhang, X., Ji, B., Yao, H., Ling, X., Guo, Z., Deng, H., Wu, X."Yifuning postpones ovarian aging through antioxidant mechanisms and suppression of the Rb/p53 signal transduction pathway". Molecular Medicine Reports 14, no. 1 (2016): 888-896. https://doi.org/10.3892/mmr.2016.5322