Reduction of dynamin 1 in the hippocampus of aged mice is associated with the decline in hippocampal‑dependent memory

Yoo,Dae Young; Jung,Hyo Young; Kim,Jong Whi; Yim,Hee Sun; Kim,Dae Won; Nam,Hajin; Suh,Jun Gyo; Choi,Jung Hoon; Won,Moo‑Ho; Yoon,Yeo Sung; Hwang,In Koo

doi:10.3892/mmr.2016.5804

Reduction of dynamin 1 in the hippocampus of aged mice is associated with the decline in hippocampal‑dependent memory

Authors:
- Dae Young Yoo
- Hyo Young Jung
- Jong Whi Kim
- Hee Sun Yim
- Dae Won Kim
- Hajin Nam
- Jun Gyo Suh
- Jung Hoon Choi
- Moo‑Ho Won
- Yeo Sung Yoon
- In Koo Hwang
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Affiliations: Department of Anatomy and Cell Biology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea, Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Kangneung‑Wonju National University, Gangneung, Gangwon 25457, Republic of Korea, Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea
Published online on: October 5, 2016 https://doi.org/10.3892/mmr.2016.5804
Pages: 4755-4760

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Abstract

Dynamin 1 is a known synaptic protein, which has is key in the presynaptic regulation of endocytosis. The present study investigated the association between age and the observed changes in Morris water maze performance, and immunoreactivity and protein levels of dynamin 1 in the mouse hippocampal formation. In addition, the effects of dynasore, an inhibitor of dynamin 1, on the hippocampal dependent memory were determined to elucidate the correlation between dynamin 1 and memory. In the training phase of the Morris water maze task, the mean escape latency of the aged group (24 months old) was significantly longer, compared with that of the adult group (4 months old), although the average swimming speed and the total distance traveled during the probe trial were similar in the two groups. In the aged group, the time spent locating the target platform was significantly longer and the time spent in the correct quadrant was significantly shorter, compared with those in the adult group. In the adult group, a moderate level of dynamin 1 was detected in the hippocampal CA1 and CA3 regions, and in the dentate gyrus. In the aged group, the immunoreactivity of dynamin 1 was almost eliminated in the CA3 region and the dentate gyrus. In addition, the protein levels of dynamin 1 in the brain were significantly lower in the aged group, compared with those in the adult group. The direct infusion of dynasore, significantly reduced the contextual memory, compared with that of animals in the vehicle‑treated group. These results suggested that dynamin 1 was susceptible to the aging process, and that a reduction in dynamin 1 may result in hippocampal‑dependent memory deficits by disrupting endocytosis and the release of neurotransmitters.

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