Danggui‑Sayuk‑Ga‑Osuyu‑Senggang‑Tang ameliorates cold‑induced vasoconstriction in vitro and in vivo

  • Authors:
    • Kangwook Lee
    • Sung‑Gook Cho
    • Sang‑Mi Woo
    • Ah Jeong Kim
    • Kang Min Lee
    • Ho Yeon Go
    • Seung‑Ho Sun
    • Tae‑Hun Kim
    • Ki‑Yong Jung
    • You‑Kyung Choi
    • Eun Mee Lim
    • Yun‑Kyung Song
    • Jong‑Hyeong Park
    • Chan‑Yong Jun
    • Seong‑Gyu Ko
  • View Affiliations

  • Published online on: October 5, 2016     https://doi.org/10.3892/mmr.2016.5805
  • Pages: 4723-4728
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Danggui-Sayuk-Ga-Osuyu-Senggang-Tang (DSGOST), one of the traditional Chinese medicines, has long been prescribed for patients suffering from Raynaud phenomenon (RP) in Northeast Asian countries, including China, Japan and Korea. Although a previous in vitro study from our laboratory revealed that DSGOST prevents cold (25˚C)‑induced RhoA activation and endothelin‑1 (ET‑1) production in endothelial cells (ECs), the mechanisms by which DSGOST is able to alleviate the symptoms of RP have yet to be fully elucidated. The present study aimed to demonstrate that DSGOST regulates RhoA‑mediated pathways in cold‑exposed pericytes. In pericytes, DSGOST amplified cold‑induced RhoA activation, while markedly reducing ET‑1‑induced RhoA activation. Additionally, DSGOST‑mediated regulation of RhoA was closely associated with Rho‑associated, coiled‑coil‑containing protein kinase 1 (ROCK1)/testis‑specific kinase 1 (TESK1)/PDXP, but not with LIM domain kinase 1/2 (LIMK1/2), cofilin and myosin light chain (MLC). Thus, DSGOST activation of RhoA/ROCK1/TESK1/PDXP in cold‑exposed pericytes appeared to be crucial for treating vessel contraction. In addition, the DSGOST effect on the RhoA‑mediated pathway in cold‑induced human umbilical vein endothelial cells or human dermal microvascular endothelial cells was similar to that in ET‑1‑treated pericytes, but not in cold‑induced pericytes. The results of the present study further confirmed that DSGOST inhibits cold‑induced contraction of the mouse tail vein in vivo. Furthermore, DSGOST treatment reduced cold‑induced expression of the α2c‑adrenergic receptor in mouse tail vessels. Therefore, the data in the present study suggest that DSGOST may be useful for the treatment of RP‑like disease.
View Figures
View References

Related Articles

Journal Cover

November-2016
Volume 14 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lee K, Cho SG, Woo SM, Kim AJ, Lee KM, Go HY, Sun SH, Kim TH, Jung KY, Choi YK, Choi YK, et al: Danggui‑Sayuk‑Ga‑Osuyu‑Senggang‑Tang ameliorates cold‑induced vasoconstriction in vitro and in vivo. Mol Med Rep 14: 4723-4728, 2016.
APA
Lee, K., Cho, S., Woo, S., Kim, A.J., Lee, K.M., Go, H.Y. ... Ko, S. (2016). Danggui‑Sayuk‑Ga‑Osuyu‑Senggang‑Tang ameliorates cold‑induced vasoconstriction in vitro and in vivo. Molecular Medicine Reports, 14, 4723-4728. https://doi.org/10.3892/mmr.2016.5805
MLA
Lee, K., Cho, S., Woo, S., Kim, A. J., Lee, K. M., Go, H. Y., Sun, S., Kim, T., Jung, K., Choi, Y., Lim, E. M., Song, Y., Park, J., Jun, C., Ko, S."Danggui‑Sayuk‑Ga‑Osuyu‑Senggang‑Tang ameliorates cold‑induced vasoconstriction in vitro and in vivo". Molecular Medicine Reports 14.5 (2016): 4723-4728.
Chicago
Lee, K., Cho, S., Woo, S., Kim, A. J., Lee, K. M., Go, H. Y., Sun, S., Kim, T., Jung, K., Choi, Y., Lim, E. M., Song, Y., Park, J., Jun, C., Ko, S."Danggui‑Sayuk‑Ga‑Osuyu‑Senggang‑Tang ameliorates cold‑induced vasoconstriction in vitro and in vivo". Molecular Medicine Reports 14, no. 5 (2016): 4723-4728. https://doi.org/10.3892/mmr.2016.5805