Molecular inhibition mechanisms of cell migration and invasion by coix polysaccharides in A549 NSCLC cells via targeting S100A4

Luo,Cheng; Wang,Xin; An,Can; Hwang,Chin‑Fa; Miao,Wenhua; Yang,Lu; Xu,Maonian; Bai,Aiping; Deng,Shanggui

doi:10.3892/mmr.2016.5985

Molecular inhibition mechanisms of cell migration and invasion by coix polysaccharides in A549 NSCLC cells via targeting S100A4

Authors:
- Cheng Luo
- Xin Wang
- Can An
- Chin‑Fa Hwang
- Wenhua Miao
- Lu Yang
- Maonian Xu
- Aiping Bai
- Shanggui Deng
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Affiliations: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, P.R. China, Department of Food Science and Technology, Hung Kuang University, Taichung 43302, Taiwan, R.O.C., Division of Pharmacognosy, Department of Medicinal Chemistry, Biomedical Center, University of Uppsala, 75123 Uppsala, Sweden, Department of Food and Environmental Sciences, Division of Food Chemistry, University of Helsinki, F‑00014 Helsinki, Finland, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02215, USA
Published online on: December 5, 2016 https://doi.org/10.3892/mmr.2016.5985
Pages: 309-316

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Abstract

S100 calcium binding protein A4 (S100A4) promotes extracellular signal transduction, intercellular adhesion, motility and mobility. Different extracts from Coix lachryma-jobi have been used for the treatment of various types of cancer in Asia. In our previous study, the polysaccharide fraction extact, CP1, induced cell apoptosis of non‑small cell lung cancer cells. In the current study, CP1 inhibited migration and invasion of A549 cells in a scratch wound healing assay and matrigel invasion assay, respectively. Furthermore, reverse transcription‑polymerase chain reaction and western blotting demonstrated that CP1 downregulated the gene and protein expression levels of S100A4. In silico docking analysis demonstrated that polysaccharides may not interfere with dimerization, whereas, the affinity of polysaccharides for an S100A4‑NMIIA pocket was margnially greater than at the dimerization sites. Thus, CP1 inhibited A549 cell migration and invasion potentially via downregulation of S100A4, and may also interact with the binding site of S100A4‑NMIIA, which indicated that CP1 has potential as an alternative cancer chemotherapeutic by targeting S100A4.

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