cPKCγ membrane translocation is involved in herkinorin‑induced neuroprotection against cerebral ischemia/reperfusion injury in mice

Gui,Xiaochen; Cui,Xu; Wei,Haiping; Feng,Guang; Zhang,Xuezheng; He,Yongjin; Li,Junfa; Li,Tianzuo

doi:10.3892/mmr.2016.5995

cPKCγ membrane translocation is involved in herkinorin‑induced neuroprotection against cerebral ischemia/reperfusion injury in mice

Authors:
- Xiaochen Gui
- Xu Cui
- Haiping Wei
- Guang Feng
- Xuezheng Zhang
- Yongjin He
- Junfa Li
- Tianzuo Li
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Affiliations: Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China, Department of Neurobiology, Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, P.R. China, Department of Anesthesiology, Tianjin Hospital, Tianjin 300211, P.R. China
Published online on: December 6, 2016 https://doi.org/10.3892/mmr.2016.5995
Pages: 221-227
Copyright: © Gui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Herkinorin is an opiate analgesic with limited adverse effects, functioning as a primary selective atypical opioid µ agonist. The present study aimed to identify whether herkinorin has a positive effect on ischemic/reperfusion (I/R) injury. Adult male C57BL/6 mice were randomly divided into five groups: i) Naïve, ii) sham, iii) I/R, iv) I/R with dimethyl sulfoxide (I/R+D) and v) I/R with herkinorin (I/R+H). The I/R injury model was induced by occluding the middle cerebral artery for 1 h followed by 24 h or 7 days of reperfusion. Neurobehavioral scores and sensorimotor functions were examined 24 h and 7 days following reperfusion. In addition, infarct volumes were examined at these time points using a 2,3,5‑triphenyltetrazolium chloride assay. Herkinorin treatment improved neurobehavioral and sensorimotor functional recovery from I/R‑induced brain injury. There was a significant decrease in infarct volume in the I/R+H group at 24 h or 7 days following reperfusion compared with the I/R and I/R+D groups. Western blotting suggested that the decrease in conventional protein kinase C γ (cPKCγ) membrane translocation in the peri‑infarct region may be attenuated by herkinorin pretreatment. These results indicated that herkinorin may be beneficial in I/R‑induced mouse brain injury, and this may be attributed to the membrane translocation of cPKCγ following activation.

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