Tissue inhibitor of metalloproteinases 1, a novel biomarker of tuberculosis

Chen,Yingyu; Wang,Jieru; Ge,Pan; Cao,Dejun; Miao,Beiping; Robertson,Ian; Zhou,Xia; Zhang,Li; Chen,Huanchun; Guo,Aizhen

doi:10.3892/mmr.2016.5998

Tissue inhibitor of metalloproteinases 1, a novel biomarker of tuberculosis

Authors:
- Yingyu Chen
- Jieru Wang
- Pan Ge
- Dejun Cao
- Beiping Miao
- Ian Robertson
- Xia Zhou
- Li Zhang
- Huanchun Chen
- Aizhen Guo
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Affiliations: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R. China, China Australia Joint Research and Training Center for Veterinary Epidemiology, Wuhan, Hubei 430070, P.R. China, Tuberculosis Department, Wuhan Medical Treatment Center, Wuhan, Hubei 430023, P.R. China
Published online on: December 7, 2016 https://doi.org/10.3892/mmr.2016.5998
Pages: 483-487

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Abstract

Tuberculosis (TB) is an important infectious disease of humans and other animals. Conventional diagnostic methods, including the tuberculin skin test, chest X‑rays and bacterial culture, have certain innate disadvantages for the early, rapid and specific diagnosis of tuberculosis. The present study aimed to identify a novel diagnostic biomarker to overcome these disadvantages. The potential target identified in the present study was tissue inhibitor of metalloproteinases 1 (TIMP‑1), which has previously been demonstrated to be critical in the immune response to TB. The concentration of TIMP‑1 in the blood was determined using a commercial ELISA kit, and the relative mRNA expression levels following bacterial infection were detected by reverse transcription‑quantitative polymerase chain reaction. Based on a clinical and microbiological diagnosis, the ELISA for plasma TIMP‑1 had a sensitivity of 91.80% [95% confidence interval (CI): 85.44, 96.00] and a specificity of 91.41% (95% CI: 85.14, 95.63). In a THP‑1 cell model, Bacillus Calmette‑Guérin and Mycobacterium bovis significantly upregulated the mRNA expression levels of TIMP‑1 post infection in a time‑dependent manner (P=0.006 for BCG 24 h PI, P=3.2x10‑7 for M. bovis 24 PI). The results of the present study indicate that plasma TIMP‑1 may be a potential biomarker for the diagnosis of TB.

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