Mechanical stress influences the viability and morphology of human parametrial ligament fibroblasts

Hu,Ming; Hong,Li; Hong,Shasha; Min,Jie; Zhao,Yang; Yang,Qing; Zhang,Qifan; Tang,Jianming; Li,Yang

doi:10.3892/mmr.2016.6052

Mechanical stress influences the viability and morphology of human parametrial ligament fibroblasts

Authors:
- Ming Hu
- Li Hong
- Shasha Hong
- Jie Min
- Yang Zhao
- Qing Yang
- Qifan Zhang
- Jianming Tang
- Yang Li
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Affiliations: Department of Gynaecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
Published online on: December 15, 2016 https://doi.org/10.3892/mmr.2016.6052
Pages: 853-858

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Abstract

The present study aimed to investigate damage to human parametrial ligament fibroblasts by detecting cell proliferation, cytoskeletal structure, cellular alterations and senescence. Uterosacral and cardinal ligaments were obtained from 10 patients with cervical intraepithelial neoplasia grade II‑III, who had received total vaginal hysterectomies, and fibroblasts were derived from this tissue. Fibroblasts were stretched using a four‑point bending system with a force of 0 (control), 1,333 µ strain (1 mm) or 5,333 µ strain (4 mm) for 4 h. The present study revealed that mechanical force significantly reduced cell proliferation and increased cell senescence. As mechanical force increased, the mitochondria of fibroblasts began to exhibit vacuolization, and the cell cytoskeleton began to depolymerize and rearrange. In conclusion, the present study demonstrated that mechanical forces within a certain range may induce cell damage via mitochondrial injury, cytoskeletal alterations and increased cell senescence, resulting in decreased cell viability of pelvic fibroblasts.

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