Effects of Lycium barbarum polysaccharides on the damage to human endometrial stromal cells induced by hydrogen peroxide

Shan,Tieying; Shan,Tieqiang; Liu,Fengli; Zheng,Haiping; Li,Guiying

doi:10.3892/mmr.2016.6080

Effects of Lycium barbarum polysaccharides on the damage to human endometrial stromal cells induced by hydrogen peroxide

Authors:
- Tieying Shan
- Tieqiang Shan
- Fengli Liu
- Haiping Zheng
- Guiying Li
View Affiliations

Affiliations: Department of Gynaecology and Obstetrics, Affiliated Hospital of Hebei Engineering University, Handan, Hebei 056002, P.R. China, Clinical Laboratory, Qinhuangdao Harbor Hospital, Qinhuangdao, Hebei 066000, P.R. China, Department of Nephrology, Affiliated Hospital of Hebei Engineering University, Handan, Hebei 056002, P.R. China
Published online on: December 28, 2016 https://doi.org/10.3892/mmr.2016.6080
Pages: 879-884

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Previous studies have shown that Lycium barbarum polysaccharides (LBPs) serve an important role in antioxidant activity to protect the cells and tissues. However, the specific mechanism of LBPs in the prevention of endometrial damage remains to be elucidated. Using morphological observation, cell proliferation assay, the detection of superoxide dismutase (SOD) activity and the content of malondialdehyde (MDA) in cell culture supernatant fluid, the detection by western blot analysis and reverse transcription-quantitative polymerase chain reaction of the mRNA and protein expression levels of caspase‑3 and Bcl‑2 in endometrial stromal cells (ESCs), it was demonstrated that, in vitro, hydrogen peroxide (H2O2)-induced death of ESCs, increased the content of MDA and decreased the activity of SOD, and decreased the expression of Bcl-2 and increased the expression of caspase‑3. LBPs can inhibit H2O2‑induced cell death of ESCs, decrease the content of MDA in ESCs and increase the activity of SOD, as well as increasing the expression of Bcl‑2 and decreasing the expression levels of caspase‑3. These findings suggested that LBPs can inhibit H2O2‑induced apoptosis of EECs and that LBPs are able to offer a significant protection against oxidative stress to ESCs.

View Figures

View References

1	Xie JH, Dong CJ, Nie SP, Li F, Wang ZJ, Shen MY and Xie MY: Extraction, chemical composition and antioxidant activity of flavonoids from Cyclocarya paliurus (Batal.) Iljinskaja leaves. Food Chem. 186:97–105. 2015. View Article : Google Scholar : PubMed/NCBI
2	Wang ZJ, Xie JH, Kan LJ, Wang JQ, Shen MY, Li WJ, Nie SP and Xie MY: Sulfated polysaccharides from Cyclocarya paliurus reduce H2O2-induced oxidative stress in RAW264.7 cells. Int J Biol Macromol:. 80:410–417. 2015. View Article : Google Scholar : PubMed/NCBI
3	Chang RC and So KF: Use of anti-aging herbal medicine, Lycium barbarum, against aging-associated diseases. What do we know so far? Cell Mol Neurobiol. 28:643–652. 2008. View Article : Google Scholar : PubMed/NCBI
4	Li XM, Ma YL and Liu XJ: Effect of the Lycium barbarum polysaccharides on agerelated oxidative stress in aged mice. J Ethnopharmacol. 111:504–511. 2007. View Article : Google Scholar : PubMed/NCBI
5	Mao F, Xiao B, Jiang Z, Zhao J, Huang X and Guo J: Anticancer effect of Lycium barbarum polysaccharides on colon cancer cells involves G0/G1 phase arrest. Med Oncol. 28:121–126. 2011. View Article : Google Scholar : PubMed/NCBI
6	Luo Q, Li Z, Yan J, Zhu F, Xu RJ and Cai YZ: Lycium barbarum polysaccharides induce apoptosis in human prostate cancer cells and inhibits prostate cancer growth in a xenograft mouse model of human prostate cancer. J Med Food. 12:695–703. 2009. View Article : Google Scholar : PubMed/NCBI
7	Du G, Liu L and Fang J: Experimental study on the enhancement of murine splenic lymphocyte proliferation by Lycium barbarum glycopeptide. J Huazhong Univ Sci Technolog Med Sci. 24:518–520. 2004. View Article : Google Scholar : PubMed/NCBI
8	Amagase H and Nance DM: A randomized, double-blind, placebo-controlled, clinical study of the general effects of a standardized Lycium barbarum (Goji) juice, GoChi. J Altern Complement Med. 14:403–412. 2008. View Article : Google Scholar : PubMed/NCBI
9	Potterat O: Goji (Lycium barbarum and L. chinense): Phytochemistry, pharmacology and safety in the perspective of traditional uses and recent popularity. Planta Med. 76:7–19. 2010. View Article : Google Scholar : PubMed/NCBI
10	Wu SJ, Ng LT and Lin CC: Antioxidant activities of some common ingredients of traditional Chinese medicine, Angelica sinensisLycium barbarum and Poria cocos. Phytother Res. 18:1008–1012. 2004. View Article : Google Scholar : PubMed/NCBI
11	Luo Q, Cai Y, Yan J, Sun M and Corke H: Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum. Life Sci. 76:137–149. 2004. View Article : Google Scholar : PubMed/NCBI
12	Xiao J, Liong EC, Ching YP, Chang RC, So KF, Fung ML and Tipoe GL: Lycium barbarum polysaccharides protect mice liver from carbon tetrachloride induced oxidative stress and necroinflammation. J Ethnopharmacol. 139:462–470. 2012. View Article : Google Scholar : PubMed/NCBI
13	Marland S and Marklund G: Involvement of the superoxide anion radical in the autoxidation of pyrogollol and a convenient assay for super oxide dismutase. Eur J Biochem. 47:469–474. 1974. View Article : Google Scholar : PubMed/NCBI
14	Yagi K: A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med. 15:212–215. 1976. View Article : Google Scholar : PubMed/NCBI
15	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
16	Li GR: Study on isolation Lycium barbarum polysaccharides and its effect on anti-active oxygen free radicals. Chin J Mod Appl Pharm. 12:94–96. 2002.(In Chinese).
17	Wang JH, Wang HZ, Zhang M and Zhang SH: Anti-aging function of poly saccharides from fructus lycii. J Acta Nutrimenta Sinica. 12:189–4191. 2002.(In Chinese).
18	Wu H, Guo H and Zhao R: Effect of Lycium barbarum polysaccharide on the improvement of antioxidant ability and DNA damage in NIDDM rats. Yakugaku Zasshi. 126:365–371. 2006. View Article : Google Scholar : PubMed/NCBI
19	Li XM: Protective effect of Lycium barbarum polysaccharides on streptozotocin-induced oxidative stress in rats. Int J Biol Macromol. 40:461–465. 2007. View Article : Google Scholar : PubMed/NCBI
20	Luo Q, Li Z, Huang X, Yan J, Zhang S and Cai YZ: Lycium barbarum polysaccharides: Protective effects against heat-induced damage of rat testes and H2O2-induced DNA damage in mouse testicular cells and beneficial effect on sexual behavior and reproductive function of hemicastrated rats. Life Sci. 79:613–621. 2006. View Article : Google Scholar : PubMed/NCBI
21	Lin MT and Beal MF: Mitoehondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature. 443:787–795. 2006. View Article : Google Scholar : PubMed/NCBI
22	Jelie S, Padeletti M, Kawtt SM, Higgins C, Canfield SM, Onat D, Colombo PC, Basner RC, Factor P and LeJemtel TH: Inflammation, oxidative stress, and repair capacity of the vascular endothelium in obstructive sleep apnea. Circulation. 117:2270–2278. 2008. View Article : Google Scholar : PubMed/NCBI
23	Starke PE and Farber JL: Endogenous defenses against the cytotoxicity of hydrogen peroxide in cultured rat hepatocytes. J Biol Chem. 260:86–92. 1985.PubMed/NCBI
24	Suen DF, Norris KL and Youle RJ: Mitoehondrial dynamies and apoptosis. Genes Dev. 22:1577–1590. 2008. View Article : Google Scholar : PubMed/NCBI
25	Cimmino A, Calin GA, Fabbri M, Iorio MV, Ferracin M, Shimizu M, Wojcik SE, Aqeilan RI, Zupo S, Dono M, et al: miR-15andmiR-16 induce apoptosis by targeting Bcl-2. Proc Natl Acad Sci U S A. 102:13944–13949. 2005. View Article : Google Scholar : PubMed/NCBI
26	Heath-Engel H, Chang N and Shore G: The endoplasmic reticulum in apoptosis and autophagy: Role of the Bcl-2 protein family. Oncogene. 27:6419–6433. 2008. View Article : Google Scholar : PubMed/NCBI
27	Fan TJ, Han LH, Cong RS and Liang J: Caspase family proteases and apoptosis. Acta Biochim Biophys Sin (Shanghai). 37:719–727. 2005. View Article : Google Scholar : PubMed/NCBI
28	Dirks AJ and Lecuwenburgh C: Aging and lifelong calorie restriction result in adaptations of skeletal muscle apoptosis repressor, apoptosis-inducing factor, X-linked inhibitor of apoptosis, caspase-3, and caspase-12. Free Radic Biol Med. 36:27–39. 2004. View Article : Google Scholar : PubMed/NCBI
29	Mazumder S, Plesca D and Almasan A: Caspase-3 activation is a critical determinant of genotoxie stress-induced apoptosis. Methods Mol Biol. 414:13–21. 2008.PubMed/NCBI