BDNF regulates the expression and secretion of VEGF from osteoblasts via the TrkB/ERK1/2 signaling pathway during fracture healing

  • Authors:
    • Zitao Zhang
    • Yan Zhang
    • Zhengnan Zhou
    • Hongfei Shi
    • Xusheng Qiu
    • Jin Xiong
    • Yixin Chen
  • View Affiliations

  • Published online on: January 12, 2017     https://doi.org/10.3892/mmr.2017.6110
  • Pages: 1362-1367
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Abstract

Brain‑derived neurotrophic factor (BDNF), a member of the neurotropic family, is expressed in osteoblast‑like cells of a fracture callus, however, its role in fracture healing remains to be fully elucidated. Osteoblasts isolated from Sprague Dawley rats were stimulated by BDNF in a dose‑ and time‑dependent manner. Immunoblotting and immunofluorescence was used to detect the expression and distribution of targeted proteins. The concentration of vascular endothelial growth factor (VEGF) released in medium was determined using an ELISA. PD98059 and K252a were used to investigate the signaling pathways that may be involved. The present study demonstrated that BDNF was involved in fracture repair by controlling the expression and secretion of VEGF from osteoblasts, which predominantly drives angiogenesis during fracture healing. Tropomyosin‑related kinase B (TrkB), the specific receptor of BDNF, was shown to be expressed at high levels in the osteoblasts. Following BDNF stimulation, TrkB and extracellular signal‑regulated kinase 1/2 (ERK1/2) were rapidly activated. The inhibition of TrkB by K252a decreased the expression and secretion of VEGF, and suppressed the phosphorylation level of ERK1/2. PD98059, an antagonist of ERK1/2, elicited the same effects on VEGF from the BDNF‑stimulated osteoblasts, however, it did not affect the phosphorylation of TrkB. In conclusion, during fracture healing, BDNF was found to stimulate the expression and secretion of VEGF from osteoblasts via the TrkB/ERK1/2 signaling pathway.
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March-2017
Volume 15 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang Z, Zhang Y, Zhou Z, Shi H, Qiu X, Xiong J and Chen Y: BDNF regulates the expression and secretion of VEGF from osteoblasts via the TrkB/ERK1/2 signaling pathway during fracture healing. Mol Med Rep 15: 1362-1367, 2017.
APA
Zhang, Z., Zhang, Y., Zhou, Z., Shi, H., Qiu, X., Xiong, J., & Chen, Y. (2017). BDNF regulates the expression and secretion of VEGF from osteoblasts via the TrkB/ERK1/2 signaling pathway during fracture healing. Molecular Medicine Reports, 15, 1362-1367. https://doi.org/10.3892/mmr.2017.6110
MLA
Zhang, Z., Zhang, Y., Zhou, Z., Shi, H., Qiu, X., Xiong, J., Chen, Y."BDNF regulates the expression and secretion of VEGF from osteoblasts via the TrkB/ERK1/2 signaling pathway during fracture healing". Molecular Medicine Reports 15.3 (2017): 1362-1367.
Chicago
Zhang, Z., Zhang, Y., Zhou, Z., Shi, H., Qiu, X., Xiong, J., Chen, Y."BDNF regulates the expression and secretion of VEGF from osteoblasts via the TrkB/ERK1/2 signaling pathway during fracture healing". Molecular Medicine Reports 15, no. 3 (2017): 1362-1367. https://doi.org/10.3892/mmr.2017.6110