Open Access

Knockdown of the differentially expressed gene TNFRSF12A inhibits hepatocellular carcinoma cell proliferation and migration in vitro

  • Authors:
    • Tao Wang
    • Sicong Ma
    • Xingxing Qi
    • Xiaoyin Tang
    • Dan Cui
    • Zhi Wang
    • Jiachang Chi
    • Ping Li
    • Bo Zhai
  • View Affiliations

  • Published online on: January 26, 2017     https://doi.org/10.3892/mmr.2017.6154
  • Pages: 1172-1178
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Human hepatocellular carcinoma (HCC) has been reported to be highly insensitive to conventional chemotherapy. In the current study, the Agilent Whole Human Genome Oligo Microarray (4x44 K) was used in order to identify the differentially expressed genes between HCC and adjacent tissues, and the top 22 differentially expressed genes were confirmed through reverse transcription‑quantitative polymerase chain reaction. Among the identified differences in gene expression, expression of tumor necrosis factor receptor superfamily member 12A (TNFRSF12A) was markedly higher in HCC tissue than in adjacent tissue. Previous studies have suggested that TNFRSF12A may serve a role in tumor growth and metastasis, thus in the current study, TNFRSF12A was knocked down in the SMMC7721 cell line through siRNA. This demonstrated that cells exhibited reduced reproductive and metastatic capacity ex vivo. Thus, the results of the current study suggest that TNFRSF12A may be a candidate therapeutic target for cancer including HCC, and additional genes that exhibited significantly different expression from normal adjacent tissues require further study.
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March-2017
Volume 15 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang T, Ma S, Qi X, Tang X, Cui D, Wang Z, Chi J, Li P and Zhai B: Knockdown of the differentially expressed gene TNFRSF12A inhibits hepatocellular carcinoma cell proliferation and migration in vitro. Mol Med Rep 15: 1172-1178, 2017.
APA
Wang, T., Ma, S., Qi, X., Tang, X., Cui, D., Wang, Z. ... Zhai, B. (2017). Knockdown of the differentially expressed gene TNFRSF12A inhibits hepatocellular carcinoma cell proliferation and migration in vitro. Molecular Medicine Reports, 15, 1172-1178. https://doi.org/10.3892/mmr.2017.6154
MLA
Wang, T., Ma, S., Qi, X., Tang, X., Cui, D., Wang, Z., Chi, J., Li, P., Zhai, B."Knockdown of the differentially expressed gene TNFRSF12A inhibits hepatocellular carcinoma cell proliferation and migration in vitro". Molecular Medicine Reports 15.3 (2017): 1172-1178.
Chicago
Wang, T., Ma, S., Qi, X., Tang, X., Cui, D., Wang, Z., Chi, J., Li, P., Zhai, B."Knockdown of the differentially expressed gene TNFRSF12A inhibits hepatocellular carcinoma cell proliferation and migration in vitro". Molecular Medicine Reports 15, no. 3 (2017): 1172-1178. https://doi.org/10.3892/mmr.2017.6154