Open Access

Overexpression of KAI1/CD82 suppresses in vitro cell growth, migration, invasion and xenograft growth in oral cancer

  • Authors:
    • Juan Chai
    • Liangzhi Du
    • Jun Ju
    • Chao Ma
    • Zhiyuan Shen
    • Xiangming Yang
    • Liang Liang
    • Qianwei Ni
    • Moyi Sun
  • View Affiliations

  • Published online on: February 9, 2017     https://doi.org/10.3892/mmr.2017.6186
  • Pages: 1527-1532
  • Copyright: © Chai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

KAI1/CD82 is a metastatic suppressor gene in human prostate cancer and several other types of cancer in humans. The present study aimed to examine the role of the overexpression of KAI1 in the progression of oral cancer. Human KAI1/CD82 cDNA was transfected into OSCC‑15 and 293T cell lines, and its effects on OSCC‑15 cell proliferation, invasion and apoptosis were assessed by performing a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, Matrigel invasion and Annexin V‑FITC staining, respectively. In addition, a xenograft model was used to assess the effect of KAI1/CD82 on the in vivo growth of tumors. The overexpression of KAI1/CD82 inhibited the proliferation and invasion of OSCC-15 cells. It also enhanced the apoptotic rate of the OSCC‑15 cells. Furthermore, the overexpression of KAI1/CD82 inhibited tumor growth in the xenograft model. The results demonstrated that the overexpression of KAI1/CD82 significantly inhibited the proliferation and invasion of human oral cancer cells, and inhibited tumor growth in the xenograft model. Therefore, KAI1/CD82 may be considered as a potential therapeutic target in oral cancer.
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April-2017
Volume 15 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Chai J, Du L, Ju J, Ma C, Shen Z, Yang X, Liang L, Ni Q and Sun M: Overexpression of KAI1/CD82 suppresses in vitro cell growth, migration, invasion and xenograft growth in oral cancer. Mol Med Rep 15: 1527-1532, 2017
APA
Chai, J., Du, L., Ju, J., Ma, C., Shen, Z., Yang, X. ... Sun, M. (2017). Overexpression of KAI1/CD82 suppresses in vitro cell growth, migration, invasion and xenograft growth in oral cancer. Molecular Medicine Reports, 15, 1527-1532. https://doi.org/10.3892/mmr.2017.6186
MLA
Chai, J., Du, L., Ju, J., Ma, C., Shen, Z., Yang, X., Liang, L., Ni, Q., Sun, M."Overexpression of KAI1/CD82 suppresses in vitro cell growth, migration, invasion and xenograft growth in oral cancer". Molecular Medicine Reports 15.4 (2017): 1527-1532.
Chicago
Chai, J., Du, L., Ju, J., Ma, C., Shen, Z., Yang, X., Liang, L., Ni, Q., Sun, M."Overexpression of KAI1/CD82 suppresses in vitro cell growth, migration, invasion and xenograft growth in oral cancer". Molecular Medicine Reports 15, no. 4 (2017): 1527-1532. https://doi.org/10.3892/mmr.2017.6186