Open Access

miR-34a is downregulated in human osteosarcoma stem-like cells and promotes invasion, tumorigenic ability and self-renewal capacity

Retraction in: /10.3892/mmr.2024.13196

  • Authors:
    • Yonggen Zou
    • Yuanshuai Huang
    • Jiexiang Yang
    • Jian Wu
    • Cheng Luo
  • View Affiliations

  • Published online on: February 9, 2017     https://doi.org/10.3892/mmr.2017.6187
  • Pages: 1631-1637
  • Copyright: © Zou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNA-34 (miR-34), in particular miR-34a, has a negative regulatory effect on osteosarcoma cell proliferation, migration and invasion. Notably, it is also a post‑transcriptional regulatory factor of (sex determining region Y)‑box 2 (Sox-2), which is required for osteosarcoma cell self‑renewal and tumorigenesis. As a direct regulator of Sox‑2, miR‑34a has been hypothesized to be greatly associated with the regulation of malignancies in osteosarcoma. To investigate the role of miR-34a in the malignancies of osteosarcoma, reverse transcription‑quantitative polymerase chain reaction was performed to detect the expression level of miR‑34a in osteospheres. The results revealed that the miR‑34a, b and c were suppressed in osteosarcoma stem‑like cells (OSCs) and osteospheres. The introduction of miR‑34a mimics and short hairpin (sh)RNA targeting Sox‑2 mRNA (shSox‑2) in human OSCs markedly reduced their transformation properties in vitro and their capacity to form tumors in soft agar. Furthermore, the epigenetic expression of miR‑34a and shSox‑2 inhibited the expression of the stem cell marker, stem cell antigen‑1 and led to the failure of osteosphere formation, respectively. The data of the present study indicated that the inhibitory role of miR‑34a on tumor growth and metastasis of osteosarcoma may function by reducing the maintenance of osteosphere self‑renewal capacity, elimination of tumorigenic ability and invasion of osteosarcoma in vitro. These findings may provide the basis for a novel therapeutic target of osteosarcomas based on inducing the expression of miR-34a.
View Figures
View References

Related Articles

Journal Cover

April-2017
Volume 15 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zou Y, Huang Y, Yang J, Wu J and Luo C: miR-34a is downregulated in human osteosarcoma stem-like cells and promotes invasion, tumorigenic ability and self-renewal capacity Retraction in /10.3892/mmr.2024.13196. Mol Med Rep 15: 1631-1637, 2017
APA
Zou, Y., Huang, Y., Yang, J., Wu, J., & Luo, C. (2017). miR-34a is downregulated in human osteosarcoma stem-like cells and promotes invasion, tumorigenic ability and self-renewal capacity Retraction in /10.3892/mmr.2024.13196. Molecular Medicine Reports, 15, 1631-1637. https://doi.org/10.3892/mmr.2017.6187
MLA
Zou, Y., Huang, Y., Yang, J., Wu, J., Luo, C."miR-34a is downregulated in human osteosarcoma stem-like cells and promotes invasion, tumorigenic ability and self-renewal capacity Retraction in /10.3892/mmr.2024.13196". Molecular Medicine Reports 15.4 (2017): 1631-1637.
Chicago
Zou, Y., Huang, Y., Yang, J., Wu, J., Luo, C."miR-34a is downregulated in human osteosarcoma stem-like cells and promotes invasion, tumorigenic ability and self-renewal capacity Retraction in /10.3892/mmr.2024.13196". Molecular Medicine Reports 15, no. 4 (2017): 1631-1637. https://doi.org/10.3892/mmr.2017.6187