Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation

  • Authors:
    • Yong‑Xian Guo
    • Liang Liu
    • Dong‑Zhan Yan
    • Jian‑Ping Guo
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  • Published online on: February 22, 2017     https://doi.org/10.3892/mmr.2017.6234
  • Pages: 2333-2338
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Abstract

Osteoarthritis (OA) is an inflammatory disorder dealing with the focal degradation of articular cartilage. Oxidative stress and inflammation are the major events in OA. The present study aimed at identifying the mechanism of the potent antioxidant, plumbagin, in protecting against hydrogen peroxide (H2O2)‑induced chondrocyte oxidative stress and inflammatory signaling. Oxidative stress was determined by measuring reactive oxygen species, lipid peroxidation, non‑enzymic (glutathione; GSH) and enzymic antioxidant activities (GSH, glutathione S‑transferase, glutathione peroxidase, superoxide dismutase, catalase). Expression levels of nuclear factor (erythroid‑derived 2)‑like 2 (Nrf‑2), heme oxygenase 1 (HO‑1), NAD(P)H:quinone oxidoreductase 1 (NQO‑1), nuclear factor‑κB (NF‑κB), cyclooxygenase‑2 (COX‑2) and inducible NO synthase (iNOS) were determined by western blot analysis. Pro‑inflammatory cytokine expression levels were assessed using ELISA. Results from reactive oxygen species generation, lipid peroxidation content and antioxidant enzyme activities demonstrated that plumbagin significantly inhibited oxidative stress status in H2O2‑induced chondrocytes. In addition, plumbagin modulated transcription factors involved in redox and inflammation regulation, including NF‑κB and Nrf‑2, by nuclear expression. plumbagin enhanced antioxidant status by increasing the expression levels of Nrf‑2 target genes, including HO‑1 and NQO‑1. An anti‑inflammatory effect against chondrocyte‑induced inflammation was demonstrated by downregulating COX‑2, iNOS and pro‑inflammatory cytokine expression levels (tumor necrosis factor‑α, interleukin (IL)‑6 and IL‑8). The present study identified strong evidence for a protective role of plumbagin against H2O2-induced oxidative stress and inflammation in chondrocytes by modulating redox signaling transcription factors.
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April-2017
Volume 15 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Guo YX, Liu L, Yan DZ and Guo JP: Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation. Mol Med Rep 15: 2333-2338, 2017
APA
Guo, Y., Liu, L., Yan, D., & Guo, J. (2017). Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation. Molecular Medicine Reports, 15, 2333-2338. https://doi.org/10.3892/mmr.2017.6234
MLA
Guo, Y., Liu, L., Yan, D., Guo, J."Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation". Molecular Medicine Reports 15.4 (2017): 2333-2338.
Chicago
Guo, Y., Liu, L., Yan, D., Guo, J."Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation". Molecular Medicine Reports 15, no. 4 (2017): 2333-2338. https://doi.org/10.3892/mmr.2017.6234