The role of ZFP580, a novel zinc finger protein, in TGF-mediated cytoprotection against chemical hypoxia‑induced apoptosis in H9c2 cardiac myocytes

Mao,Shi‑Yun; Meng,Xiang‑Yan; Xu,Zhong‑Wei; Zhang,Wen‑Cheng; Jin,Xiao‑Han; Chen,Xi; Zhou,Xin; Li,Yu‑Ming; Xu,Rui‑Cheng

doi:10.3892/mmr.2017.6236

The role of ZFP580, a novel zinc finger protein, in TGF-mediated cytoprotection against chemical hypoxia‑induced apoptosis in H9c2 cardiac myocytes

Authors:
- Shi‑Yun Mao
- Xiang‑Yan Meng
- Zhong‑Wei Xu
- Wen‑Cheng Zhang
- Xiao‑Han Jin
- Xi Chen
- Xin Zhou
- Yu‑Ming Li
- Rui‑Cheng Xu
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Affiliations: Sichuan Provincial Corps Hospital, Chinese People's Armed Police Forces, Leshan, Sichuan 614000, P.R. China, Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Tianjin 300309, P.R. China, Central Laboratory, Logistics University of Chinese People's Armed Police Force, Tianjin 300309, P.R. China, Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, P.R. China, Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Force, Tianjin 300309, P.R. China
Published online on: February 22, 2017 https://doi.org/10.3892/mmr.2017.6236
Pages: 2154-2162
Copyright: © Mao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Zing finger protein 580 (ZFP580) is a novel Cys2-His2 zinc-finger transcription factor that has an anti-apoptotic role in myocardial cells. It is involved in the endothelial transforming growth factor‑β1 (TGF‑β1) signal transduction pathway as a mothers against decapentaplegic homolog (Smad)2 binding partner. The aim of the present study was to determine the involvement of ZFP580 in TGF‑β1‑mediated cytoprotection against chemical hypoxia‑induced apoptosis, using H9c2 cardiac myocytes. Hypoxia was chemically induced in H9c2 myocardial cells by exposure to cobalt chloride (CoCl2). In response to hypoxia, cell viability was decreased, whereas the expression levels of hypoxia inducible factor-1α and ZFP580 were increased. Pretreatment with TGF‑β1 attenuated CoCl2‑induced cell apoptosis and upregulated ZFP580 protein expression; however, these effects could be suppressed by SB431542, an inhibitor of TGF‑β type I receptor and Smad2/3 phosphorylation. Furthermore, suppression of ZFP580 expression by RNA interference reduced the anti‑apoptotic effects of TGF‑β1 and thus increased CoCl2‑induced apoptosis. B‑cell lymphoma (Bcl)‑2‑associated X protein/Bcl‑2 ratio, reactive oxygen species generation and caspase‑3 activation were also increased following ZFP580 inactivation. In conclusion, these results indicate that ZFP580 is a component of the TGF-β1/Smad signaling pathway, and is involved in the protective effects of TGF‑β1 against chemical hypoxia‑induced cell apoptosis, through inhibition of the mitochondrial apoptotic pathway.

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