Open Access

Generation and characterization of induced pluripotent stem cells from guinea pig fetal fibroblasts

Retraction in: /10.3892/mmr.2019.10020

  • Authors:
    • Yuehong Wu
    • Ouyang Li
    • Chengwen He
    • Yong Li
    • Min Li
    • Xiaoming Liu Liu
    • Yujiong Wang
    • Yulong He
  • View Affiliations

  • Published online on: April 4, 2017     https://doi.org/10.3892/mmr.2017.6431
  • Pages: 3690-3698
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Induced pluripotent stem cells (iPS) represent an important tool to develop disease‑modeling assays, drug testing assays and cell‑based replacement therapies. The application of iPS in these fields requires the development of suitable animal models. Of the suitable species, guinea pigs are particularly important and offer significant advantages. Successful iPS generation has been accomplished in a number of species; however, it has not been reported in the guinea pig. The present study successfully generated iPS from guinea pigs (giPS) using single polycistronic virus transduction with mouse octamer‑binding transcription factor 4 (Oct4), sex determining region Y‑box 2 (Sox2), Kruppel‑like factor 4 and c‑Myc. The giPS cell lines were cultured in media containing leukemia inhibitory factor and guinea pig fibroblast cells were used as feeder cells. These cultures were expanded under feeder‑free culture conditions using ESGRO Complete Plus Clonal Grade medium containing 15% fetal bovine serum on gelatin‑coated dishes. The resultant cells had a normal karyotype, exhibited alkaline phosphatase activity and expressed the pluripotency markers Oct4, Sox2 and Nanog. The cells differentiated in vivo to form teratomas that contained all three germ layers of the tissue cells. The generation of giPS may facilitate future studies investigating the mechanisms underlying innate immunity, particularly for tuberculosis. These experiments provide proof of principle that iPS technology may be adapted to use the guinea pig as a model of human diseases.
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June-2017
Volume 15 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wu Y, Li O, He C, Li Y, Li M, Liu X, Wang Y and He Y: Generation and characterization of induced pluripotent stem cells from guinea pig fetal fibroblasts Retraction in /10.3892/mmr.2019.10020. Mol Med Rep 15: 3690-3698, 2017.
APA
Wu, Y., Li, O., He, C., Li, Y., Li, M., Liu, X. ... He, Y. (2017). Generation and characterization of induced pluripotent stem cells from guinea pig fetal fibroblasts Retraction in /10.3892/mmr.2019.10020. Molecular Medicine Reports, 15, 3690-3698. https://doi.org/10.3892/mmr.2017.6431
MLA
Wu, Y., Li, O., He, C., Li, Y., Li, M., Liu, X., Wang, Y., He, Y."Generation and characterization of induced pluripotent stem cells from guinea pig fetal fibroblasts Retraction in /10.3892/mmr.2019.10020". Molecular Medicine Reports 15.6 (2017): 3690-3698.
Chicago
Wu, Y., Li, O., He, C., Li, Y., Li, M., Liu, X., Wang, Y., He, Y."Generation and characterization of induced pluripotent stem cells from guinea pig fetal fibroblasts Retraction in /10.3892/mmr.2019.10020". Molecular Medicine Reports 15, no. 6 (2017): 3690-3698. https://doi.org/10.3892/mmr.2017.6431