Expression of reproductive hormone receptors and contraction‑associated genes in porcine uterus during the estrous cycle

An,Sung‑Min; Kim,Sun  Suk; Kim,Jun; Park,Mee‑Na; Lee,Jae‑Eon; Cho,Seong  Keun; Lee,Kyu‑Sup; An,Beum‑Soo

doi:10.3892/mmr.2017.6518

Expression of reproductive hormone receptors and contraction‑associated genes in porcine uterus during the estrous cycle

Authors:
- Sung‑Min An
- Sun Suk Kim
- Jun Kim
- Mee‑Na Park
- Jae‑Eon Lee
- Seong Keun Cho
- Kyu‑Sup Lee
- Beum‑Soo An
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Affiliations: Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang, Gyeongsangnam‑do 50463, Republic of Korea, Department of Obstetrics and Gynecology, Biomedical Research Institute, Pusan National University School of Medicine, Pusan, Gyeongsangnam‑do 49241, Republic of Korea, University of Ulsan College of Medicine, Seoul, Gyeonggi‑do 05505, Republic of Korea, Department of Animal Science, College of Natural Resources and Life Science, Pusan National University, Miryang, Gyeongsangnam‑do 50463, Republic of Korea
Published online on: April 27, 2017 https://doi.org/10.3892/mmr.2017.6518
Pages: 4176-4184

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Abstract

Contraction of uterus tissue frequently occurs throughout the estrous cycle and is regulated by several endogenous factors, including estradiol, progesterone, luteinizing hormone, follicle‑stimulating hormone, oxytocin (OXT) and contraction‑associated proteins (CAPs). Contraction activity of uterus tissue according to the estrous cycle is important, due to the fact that it is directly associated with balanced implantation and stable pregnancy. However, few studies have examined the mechanism of uterus contraction activity in a porcine model. In the current study, porcine uterus tissue was separated into the follicular and luteal phases by histological analysis. To investigate regulation of contraction‑associated factors according to the estrous cycle, mRNA and protein expression levels of reproductive hormonal receptors, including estrogen receptors, progesterone receptor and luteinizing hormone/choriogonadotropin receptor in addition to CAPs including OXT, OXT receptor (OXTR), hydroxyprostaglandin dehydrogenase 15‑(NAD) and gap junction α‑1 protein, were examined in the porcine uterus according to the follicular and luteal phases. For the results, hormonal receptors and CAPs were dynamically regulated depending on the estrous cycle. In conclusion, genes associated with uterine contraction and its regulatory hormonal receptors in the porcine uterus were differently regulated in the follicular and luteal phases, suggesting that these genes are critically involved in the remodeling and contraction of uterine tissue and may be required to modulate the physiological status of the uterus.

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