Schisandrin B attenuates the inflammatory response, oxidative stress and apoptosis induced by traumatic spinal cord injury via inhibition of p53 signaling in adult rats

Xin,D. Q.; Hu,Z. M.; Huo,H. J.; Yang,X. J.; Han,D.; Xing,W. H.; Zhao,Y.; Qiu,Q. H.

doi:10.3892/mmr.2017.6622

Schisandrin B attenuates the inflammatory response, oxidative stress and apoptosis induced by traumatic spinal cord injury via inhibition of p53 signaling in adult rats

Authors:
- D. Q. Xin
- Z. M. Hu
- H. J. Huo
- X. J. Yang
- D. Han
- W. H. Xing
- Y. Zhao
- Q. H. Qiu
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Affiliations: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of Spinal Surgery, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010030, P.R. China, Department of Cardiovascular Medicine, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010059, P.R. China
Published online on: May 25, 2017 https://doi.org/10.3892/mmr.2017.6622
Pages: 533-538
Copyright: © Xin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Schisandrin B is an active monomer of the Chinese magnolia vine (Schisandra chinensis) that can reduce transaminase activity in liver cells, inhibit lipid peroxidation, enhance antioxidant status, has protective effects in the liver and has antitumor effects. The present study investigated the potential protective effects of schisandrin B on the p53 signaling pathway in attenuating the inflammatory response, oxidative stress and apoptosis induced by traumatic spinal cord injury (TSCI) in adult rats. Behavioral examination, inclined plate test and spinal cord water content were used to evaluate the protective effect of schisandrin B in TSCI rats. The expression levels of superoxide dismutase (SOD), malondialdehyde (MDA), nuclear factor (NF)‑κB subunit p65 and tumor necrosis factor (TNF)‑α were examined using ELISA kits. Western blot analysis was performed to analyze the protein expression of caspase‑3 and phosphorylated (p)‑p53 in TSCI rats. In the present study, schisandrin B improved behavioral examination results and the maximum angle of inclined plate test, and inhibited spinal cord water content in rats with TSCI. Notably, schisandrin B reduced the activation of traumatic injury‑associated pathways, including SOD, MDA, NF‑κB p65 and TNF‑α, in TSCI rats. In addition, schisandrin B suppressed the TSCI‑induced expression of caspase‑3 and p‑p53 in TSCI rats. These results indicated that schisandrin B may attenuate the inflammatory response, oxidative stress and apoptosis in TSCI rats by inhibiting the p53 signaling pathway in adult rats.

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