Small protein A and phospholipase D immunization serves a protective role in a mouse pneumonia model of Acinetobacter baumannii infection

Li,Haitao; Tan,Hongyi; Hu,Yongbin; Pan,Pinhua; Su,Xiaoli; Hu,Chengpin

doi:10.3892/mmr.2017.6688

Small protein A and phospholipase D immunization serves a protective role in a mouse pneumonia model of Acinetobacter baumannii infection

Authors:
- Haitao Li
- Hongyi Tan
- Yongbin Hu
- Pinhua Pan
- Xiaoli Su
- Chengpin Hu
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Affiliations: Department of Pulmonary and Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Pathological Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
Published online on: June 6, 2017 https://doi.org/10.3892/mmr.2017.6688
Pages: 1071-1078
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acinetobacter baumannii is an important pathogen that primarily causes hospital-acquired pneumonia. The present study sought to investigate whether small protein A (SmpA) and phospholipase D (PLD) are potential candidates for protective immunity against infection with A. baumannii. Mice immunized with the fusion proteins histidine (His)‑SmpA and His‑PLD exhibited a specific immunoglobulin G response. In a pneumonia model, active and passive immunization against SmpA and PLD protected mice from A. baumannii infection. The protection was demonstrated by a markedly improved survival rate, and reduced pulmonary bacterial load, infiltration and cytokine levels in the broncho‑alveolar lavage fluid and the serum, although a combination of the two antigens did not provide improved protection compared with immunization with the individual antigens alone. In conclusion, it was identified that SmpA and PLD are highly immunogenic proteins, and potential antigen candidates for the development of effective vaccines or to prepare antisera to mitigate A. baumannii infection.

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