Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway

Wang,Di; Hu,Shuang; Zhang,Junrong; Li,Qiuyue; Liu,Xinyu; Li,Yu

doi:10.3892/mmr.2017.6745

Investigation of the neuroprotective effects of a novel synthetic compound via the mitochondrial pathway

Authors:
- Di Wang
- Shuang Hu
- Junrong Zhang
- Qiuyue Li
- Xinyu Liu
- Yu Li
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Affiliations: Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, P.R. China, School of Life Sciences, Jilin University, Changchun, Jilin 130012, P.R. China
Published online on: June 9, 2017 https://doi.org/10.3892/mmr.2017.6745
Pages: 1133-1138
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the neuroprotective effect of a novel synthetic compound (5zou) on differentiated PC12 cells against 6‑hydroxydopamine (6‑OHDA) and L‑glutamic acid (L‑Glu) neurotoxin‑induced cell injury and the potential mechanisms involved. 5zou is a 2, 2‑disubstituted 1,2‑dihydropyridine. PC12 cells were treated with 6‑OHDA and L‑Glu to establish neurotoxic cell models. MTT assay, DCFH‑DA staining, Fluo‑4‑AM staining, JC‑1 staining and western blotting were used to determine the changes in cell viability, intracellular reactive oxygen species concentration, Ca2+ influx, mitochondrial membrane potential and the protein expressions of B‑cell lymphoma‑2 (Bcl‑2) and B‑cell lymphoma‑extra large (Bcl‑xL). Morphological analysis demonstrated the effect of 5zous on neuritogenesis and differentiation in PC12 cells. The results suggested that 5zou rescued the cell viability, intracellular ROS level, Ca2+ influx, mitochondrial membrane potential, and expression of Bcl‑2 and Bcl‑xL, which were altered by 6‑OHDA and L‑Glu. The study confirmed that 5zou has neuroprotective effects on neurotoxin‑induced differentiated PC12 cells injury, potentially via the mitochondrial apoptosis pathway.

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