Scutellaria baicalensis Georgi induces caspase-dependent apoptosis via mitogen activated protein kinase activation and the generation of reactive oxygen species signaling pathways in MCF-7 breast cancer cells

  • Authors:
    • Jin Ryeong Park
    • Min Cheol Lee
    • Seong‑Cheol Moon
    • Junghoon Kim
    • Ki‑Tae Ha
    • Eun Jung Park
    • Chansik Hong
    • Byoung‑Do Seo
    • Byung Joo Kim
  • View Affiliations

  • Published online on: June 16, 2017     https://doi.org/10.3892/mmr.2017.6798
  • Pages: 2302-2308
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Scutellaria baicalensis Georgi extract (SBGE) is used in traditional herbal medicine and has also been used clinically to ameliorate the symptoms of various inflammatory diseases and cancer. In women, breast cancer is one of the most common diseases and numerous women succumb to it. The present study was undertaken to investigate the mechanism responsible for the SBGE‑induced apoptosis of MCF‑7 human breast cancer cells. SBGE was administered to cells at concentrations between 100 and 500 mg/ml, and cell viabilities were identified using an MTT assay. B‑cell lymphoma 2 (Bcl-2) and Bcl-2 X‑associated protein (Bax) family members were identified by western blotting, and the mRNA expression levels of the pro‑apoptosis genes Fas, Fas ligand (FasL) and tumor necrosis factor (TNF)‑α were assessed by reverse transcription‑polymerase chain reaction. It was identified that SBGE treatment for 24 h inhibited MCF‑7 proliferation and increased the sub‑G1 phase ratio. SBGE suppressed mitochondrial membrane potentials and SBGE‑induced apoptotic cell death was identified to be associated with downregulation of Bcl‑2, but upregulation of Bax. SBGE‑activated caspases 3 and 9, and increased reactive oxygen species generation. However, SBGE had no effect on the expression levels of Fas, FasL or TNF‑α. Furthermore, mitogen‑activated protein kinase and C‑Jun N‑terminal kinase inhibitors inhibited SBGE‑induced cell death. These results suggested that SBGE be considered as an agent for the treatment of breast cancer.
View Figures
View References

Related Articles

Journal Cover

August-2017
Volume 16 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Park JR, Lee MC, Moon SC, Kim J, Ha KT, Park EJ, Hong C, Seo BD and Kim BJ: Scutellaria baicalensis Georgi induces caspase-dependent apoptosis via mitogen activated protein kinase activation and the generation of reactive oxygen species signaling pathways in MCF-7 breast cancer cells. Mol Med Rep 16: 2302-2308, 2017.
APA
Park, J.R., Lee, M.C., Moon, S., Kim, J., Ha, K., Park, E.J. ... Kim, B.J. (2017). Scutellaria baicalensis Georgi induces caspase-dependent apoptosis via mitogen activated protein kinase activation and the generation of reactive oxygen species signaling pathways in MCF-7 breast cancer cells. Molecular Medicine Reports, 16, 2302-2308. https://doi.org/10.3892/mmr.2017.6798
MLA
Park, J. R., Lee, M. C., Moon, S., Kim, J., Ha, K., Park, E. J., Hong, C., Seo, B., Kim, B. J."Scutellaria baicalensis Georgi induces caspase-dependent apoptosis via mitogen activated protein kinase activation and the generation of reactive oxygen species signaling pathways in MCF-7 breast cancer cells". Molecular Medicine Reports 16.2 (2017): 2302-2308.
Chicago
Park, J. R., Lee, M. C., Moon, S., Kim, J., Ha, K., Park, E. J., Hong, C., Seo, B., Kim, B. J."Scutellaria baicalensis Georgi induces caspase-dependent apoptosis via mitogen activated protein kinase activation and the generation of reactive oxygen species signaling pathways in MCF-7 breast cancer cells". Molecular Medicine Reports 16, no. 2 (2017): 2302-2308. https://doi.org/10.3892/mmr.2017.6798