Gossypol induces cell death by activating apoptosis and autophagy in HT-29 cells

  • Authors:
    • Ming‑Dong Lu
    • Li‑Yi Li
    • Pi‑Hong Li
    • Tao You
    • Fei‑Hai Wang
    • Wei‑Jian Sun
    • Zhi‑Qiang Zheng
  • View Affiliations

  • Published online on: June 19, 2017     https://doi.org/10.3892/mmr.2017.6804
  • Pages: 2128-2132
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Abstract

Gossypol is a polyphenolic, yellowish compound derived from cottonseed extract. The present study examined the effects of gossypol on the apoptosis and autophagy of HT‑29 cells. A Cell Counting Kit‑8 assay, Annexin V‑FITC, JC‑1 staining and western blotting were used to identify the viability of cells, stages of apoptosis and the expression levels of the signaling proteins. Gossypol promoted apoptosis and induced the loss of mitochondrial membrane potential. Further investigation of the apoptotic mechanism revealed that gossypol increased the ratio of B‑cell lymphoma 2 (Bcl-2)-associated X protein/Bcl‑2 protein levels and upregulated the expression of caspase‑3. Gossypol also enhanced the activity of microtubule‑associated protein light chain 3 LC3‑II and Beclin‑1 and downregulated LC3‑I, in a dose‑dependent manner. Together, these finding suggested that gossypol may be a novel and potential antitumor agent.
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August-2017
Volume 16 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lu MD, Li LY, Li PH, You T, Wang FH, Sun WJ and Zheng ZQ: Gossypol induces cell death by activating apoptosis and autophagy in HT-29 cells. Mol Med Rep 16: 2128-2132, 2017.
APA
Lu, M., Li, L., Li, P., You, T., Wang, F., Sun, W., & Zheng, Z. (2017). Gossypol induces cell death by activating apoptosis and autophagy in HT-29 cells. Molecular Medicine Reports, 16, 2128-2132. https://doi.org/10.3892/mmr.2017.6804
MLA
Lu, M., Li, L., Li, P., You, T., Wang, F., Sun, W., Zheng, Z."Gossypol induces cell death by activating apoptosis and autophagy in HT-29 cells". Molecular Medicine Reports 16.2 (2017): 2128-2132.
Chicago
Lu, M., Li, L., Li, P., You, T., Wang, F., Sun, W., Zheng, Z."Gossypol induces cell death by activating apoptosis and autophagy in HT-29 cells". Molecular Medicine Reports 16, no. 2 (2017): 2128-2132. https://doi.org/10.3892/mmr.2017.6804