Open Access

Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats

  • Authors:
    • Lin Li
    • Ting Xiao
    • Fang Li
    • Yan Li
    • Ou Zeng
    • Maojun Liu
    • Biao Liang
    • Zining Li
    • Chun Chu
    • Jun Yang
  • View Affiliations

  • Published online on: June 20, 2017     https://doi.org/10.3892/mmr.2017.6813
  • Pages: 1715-1722
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to explore the effect of hydrogen sulfide (H2S) on renal tissue fibrosis and its mechanism in diabetic rats. Rats were randomly divided into four groups (n=13/group): Control group; induced diabetes mellitus group (STZ); induced diabetes mellitus treated with H2S group (STZ + H2S); normal rats treated with H2S group (H2S). The diabetic model was induced by intraperitoneal (i.p.) injections of 40 mg/kg body weight streptozotocin (STZ); the control group was treated with saline every day (i.p); NaHS (100 µmol/kg i.p.) was administered to rats of STZ + H2S group and H2S group. After 8 weeks, rat body weight and 24 h proteinuria levels were determined in each group, renal pathological morphology was analyzed by Masson's trichrome staining, collagen IV content was detected by immunohistochemistry, and periodic acid‑Schiff (PAS) staining was performed on renal glomerular and tubular basement membranes. The expression levels of matrix metalloproteinase 9 (MMP9), MMP7, tissue inhibitor of metalloproteinase 1 (TIMP1), superoxide dismutase (SOD), serine/threonine kinase AKT, transforming growth factor (TGF)‑β1, nuclear factor (NF)‑κB and several autophagy related proteins were assessed by western blot analysis. Compared with the control group, renal tissue fibrosis was observed, collagen IV expression and the 24 h proteinuria quantity was markedly increased and the amount of PAS positive material in renal glomerular and tubular basement membranes was notably increased in STZ‑treated rats. Furthermore, the expression levels of MMP9, MMP7, TIMP1, autophagy‑associated proteins, AKT, TGF‑β1 and NF‑κB protein were significantly increased, and SOD expression levels were significantly decreased in the STZ group compared with the control (P<0.05). In the H2S+STZ group, renal tissue fibrosis and the expression of collagen IV were improved, 24 h proteinuria was decreased, the amount of PAS positive material in renal glomerular and tubular basement membranes was decreased, the expression levels MMP9, MMP7, TIMP1, autophagy‑associated proteins, AKT, TGF‑β1 and NF‑κB protein were significantly decreased, and the expression levels of SOD were significantly increased compared with the STZ group (P<0.05). In conclusion, H2S may improve renal tissue fibrosis by inhibiting autophagy, upregulating SOD and downregulating AKT, TGF‑β1 and NF-κB.
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August-2017
Volume 16 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li L, Xiao T, Li F, Li Y, Zeng O, Liu M, Liang B, Li Z, Chu C, Yang J, Yang J, et al: Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats. Mol Med Rep 16: 1715-1722, 2017
APA
Li, L., Xiao, T., Li, F., Li, Y., Zeng, O., Liu, M. ... Yang, J. (2017). Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats. Molecular Medicine Reports, 16, 1715-1722. https://doi.org/10.3892/mmr.2017.6813
MLA
Li, L., Xiao, T., Li, F., Li, Y., Zeng, O., Liu, M., Liang, B., Li, Z., Chu, C., Yang, J."Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats". Molecular Medicine Reports 16.2 (2017): 1715-1722.
Chicago
Li, L., Xiao, T., Li, F., Li, Y., Zeng, O., Liu, M., Liang, B., Li, Z., Chu, C., Yang, J."Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats". Molecular Medicine Reports 16, no. 2 (2017): 1715-1722. https://doi.org/10.3892/mmr.2017.6813