Open Access

Dopamine attenuates ethanol-induced neuroapoptosis in the developing rat retina via the cAMP/PKA pathway

  • Authors:
    • Junde Han
    • Lingqi Gao
    • Jing Dong
    • Yingtian Wang
    • Mazhong Zhang
    • Jijian Zheng
  • View Affiliations

  • Published online on: June 20, 2017     https://doi.org/10.3892/mmr.2017.6823
  • Pages: 1982-1990
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Apoptosis has been identified as the primary cause of fetal alcohol spectrum disorder (FASD), and the development of methods to prevent and treat FASD have been based on the mechanisms of alcohol-induced apoptosis. The present study aimed to explore the effects of dopamine on alcohol‑induced neuronal apoptosis using whole‑mount cultures of rat retinas (postnatal day 7). Retinas were initially incubated with ethanol (100, 200 or 500 mM), and in subsequent analyses retinas were co‑incubated with ethanol (200 mM) and dopamine (10 µM). In addition, several antagonists and inhibitors were used, including a D1 dopamine receptor (D1R) antagonist (SCH23390; 10 µM), a D2R antagonist (raclopride; 40 µM), an adenosine A2A receptor (AA2AR) antagonist (SCH58261; 100 nM), an adenylyl cyclase (AC) inhibitor (SQ22536; 100 µM) and a PKA inhibitor (H‑89; 1 µM). The results demonstrated that exposure increased neuroapoptosis in the retinal ganglion cell layer (GCL) in a dose‑dependent manner. Dopamine treatment significantly attenuated ethanol‑induced neuronal apoptosis. D1R, D2R and AA2AR antagonists partially inhibited the protective effects of dopamine against ethanol‑induced apoptosis; similar results were observed with AC and PKA inhibitor treatments. In summary, the present study demonstrated that dopamine treatment may be able to attenuate alcohol‑induced neuroapoptosis in the developing rat retina by activating D1R, D2R and AA2AR, and by upregulating cyclic AMP/protein kinase A signaling.
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August-2017
Volume 16 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Han J, Gao L, Dong J, Wang Y, Zhang M and Zheng J: Dopamine attenuates ethanol-induced neuroapoptosis in the developing rat retina via the cAMP/PKA pathway. Mol Med Rep 16: 1982-1990, 2017
APA
Han, J., Gao, L., Dong, J., Wang, Y., Zhang, M., & Zheng, J. (2017). Dopamine attenuates ethanol-induced neuroapoptosis in the developing rat retina via the cAMP/PKA pathway. Molecular Medicine Reports, 16, 1982-1990. https://doi.org/10.3892/mmr.2017.6823
MLA
Han, J., Gao, L., Dong, J., Wang, Y., Zhang, M., Zheng, J."Dopamine attenuates ethanol-induced neuroapoptosis in the developing rat retina via the cAMP/PKA pathway". Molecular Medicine Reports 16.2 (2017): 1982-1990.
Chicago
Han, J., Gao, L., Dong, J., Wang, Y., Zhang, M., Zheng, J."Dopamine attenuates ethanol-induced neuroapoptosis in the developing rat retina via the cAMP/PKA pathway". Molecular Medicine Reports 16, no. 2 (2017): 1982-1990. https://doi.org/10.3892/mmr.2017.6823