Rho-kinase signaling pathway promotes the expression of PARP to accelerate cardiomyocyte apoptosis in ischemia/reperfusion

Bian,Hongjun; Zhou,Yi; Yu,Bin; Shang,Deya; Liu,Fuli; Li,Bin; Qi,Jianni

doi:10.3892/mmr.2017.6826

Rho-kinase signaling pathway promotes the expression of PARP to accelerate cardiomyocyte apoptosis in ischemia/reperfusion

Authors:
- Hongjun Bian
- Yi Zhou
- Bin Yu
- Deya Shang
- Fuli Liu
- Bin Li
- Jianni Qi
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Affiliations: Department of Emergency, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Health Care, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China, Central Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China
Published online on: June 21, 2017 https://doi.org/10.3892/mmr.2017.6826
Pages: 2002-2008
Copyright: © Bian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

It has been previously reported that Rho‑kinase (ROCK) and poly ADP-ribose polymerase (PARP) serve critical roles in myocardial ischemia/reperfusion (I/R) injury. Studies have additionally demonstrated that the activation of ROCK and the expression of PARP are increased in I/R. However, the effect and mechanism of the two proteins remains to be fully elucidated in I/R. In addition, whether they can be influenced by each other is unclear. In the present study, it was demonstrated that ischemia followed by reperfusion resulted in a significant increase in ROCK and PARP. In addition, Y‑27632 (ROCK inhibitor) and 3‑aminobenzamide (3‑AB; PARP inhibitor) pretreatment rescued myocardial infarction size and cardiomyocyte apoptosis. The inhibitory role of Y‑27632 was observed to be superior to that of the 3‑AB group. In addition, Y‑27632 and 3‑AB diminished extracellular signal‑related kinase (ERK) phosphorylation and the production of tumor necrosis factor α and interleukin 6. Overall, the results of the present study suggested that the inhibition of ROCK leads to reduced myocardial infarction size and cardiomyocyte apoptosis via the PARP/ERK signaling pathway.

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