Expression and antimicrobial character of cells transfected with human β‑defensin‑3 against periodontitis‑associated microbiota in vitro

Zhu,Minwen; Miao,Bo; Zhu,Jianhua; Wang,Haiyan; Zhou,Zengtong

doi:10.3892/mmr.2017.6913

Expression and antimicrobial character of cells transfected with human β‑defensin‑3 against periodontitis‑associated microbiota in vitro

Authors:
- Minwen Zhu
- Bo Miao
- Jianhua Zhu
- Haiyan Wang
- Zengtong Zhou
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Affiliations: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China, Department of Stomatology, Aviation General Hospital of China Medical University, Beijing 100012, P.R. China, Department of Periodontal Mucosa, Affiliated Stomatological Hospital of Jiamusi University, Jiamusi, Heilongjiang 154002, P.R. China
Published online on: July 4, 2017 https://doi.org/10.3892/mmr.2017.6913
Pages: 2455-2460
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Periodontitis is an oral chronic inflammatory disease induced by microorganisms that can destroy tooth‑supporting structures. Human β‑defensin‑3 (HBD‑3) is a type of endogenous antimicrobial peptide that inhibits a broad spectrum of microorganisms. The objectives of the present study were to transfect human periodontal ligament cells (HPDLCs) and human bone marrow stromal cells (HBMSCs) with lentivirus containing the HBD‑3 gene, determine the transfection efficiency, and investigate the antimicrobial activity of the experimental cells against periodontal pathogens. Fluorescence microscopy was used to calculated the transfection efficiency. Western blot analysis and ELISA were conducted to confirm the expression of HBD‑3 at the protein level. The effect of the HBD‑3 gene on the antimicrobial activity of the cells were demonstrated by antimicrobial tests. The results of the present study demonstrated that the transfected HPDLCs and HBMSCs stably expressed HBD‑3. In addition, periodontal pathogens and caries‑causing bacteria were susceptible to the antimicrobial activity of the cells. Both HPDLCs and HBMSCs hold potential for use as seeding cells in cell‑ and gene‑based therapies for periodontal disease. The lentiviral vector containing HBD‑3 resulted in broad‑spectrum antimicrobial activity against a variety of oral organisms, and could potentially be applied in the treatment of oral infectious diseases, including periodontitis.

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