Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3

  • Authors:
    • Yangyang Han
    • Tianxiao Wang
    • Shudong Sun
    • Zhaohui Zhai
    • Shengjian Tang
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  • Published online on: July 4, 2017     https://doi.org/10.3892/mmr.2017.6914
  • Pages: 2856-2862
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Abstract

Forkhead box L2 (FOXL2) is a transcription factor, which is involved in blepharophimosis, ptosis, and epicanthus in versus syndrome (BPES), premature ovarian failure (POF), as well as almost all stages of ovarian development and function. FOXL2 has various target genes, which are implicated in numerous processes, including sex determination, cell cycle regulation and apoptosis and stress response regulation in mammals. However, studies regarding the upstream regulation of FOXL2 are limited. In the present study, the promoter of FOXL2 was successfully cloned and registered in Gen Bank, and a dual luciferase reporter (DLR) analysis demonstrated that the luciferase activity was significantly induced by the promoter of FOXL2. Subsequently, bioinformatics analysis indicated that FOXL2 may be regulated by STAT3, and this was confirmed by a DLR analysis and western blotting, using STAT3 inhibitors. Further study using real‑time cellular analysis indicated that the viability of He La cells was markedly suppressed by STAT3 inhibitors. The present study demonstrated novel findings regarding the upstream regulation of FOXL2 expression and provide a new perspective for future studies in the field.
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September-2017
Volume 16 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Han Y, Wang T, Sun S, Zhai Z and Tang S: Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3. Mol Med Rep 16: 2856-2862, 2017.
APA
Han, Y., Wang, T., Sun, S., Zhai, Z., & Tang, S. (2017). Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3. Molecular Medicine Reports, 16, 2856-2862. https://doi.org/10.3892/mmr.2017.6914
MLA
Han, Y., Wang, T., Sun, S., Zhai, Z., Tang, S."Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3". Molecular Medicine Reports 16.3 (2017): 2856-2862.
Chicago
Han, Y., Wang, T., Sun, S., Zhai, Z., Tang, S."Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3". Molecular Medicine Reports 16, no. 3 (2017): 2856-2862. https://doi.org/10.3892/mmr.2017.6914