Protective effects of Shexiang Tongxin Dropping Pill on pituitrin‑induced acute myocardial ischemia in rats

Lin,Shan; Chu,Jianfeng; Zhang,Ling; Chen,Daxin; Xiao,Fei; Chen,Hongwei; Lin,Jiumao; Chen,Youqin; Zhu,Yuling; Peng,Jun

doi:10.3892/mmr.2017.6963

Protective effects of Shexiang Tongxin Dropping Pill on pituitrin‑induced acute myocardial ischemia in rats

Authors:
- Shan Lin
- Jianfeng Chu
- Ling Zhang
- Daxin Chen
- Fei Xiao
- Hongwei Chen
- Jiumao Lin
- Youqin Chen
- Yuling Zhu
- Jun Peng
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Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Rainbow Babies and Children's Hospital, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA, Inner Mongolia Conba Pharmaceutical Co., Ltd., Shanghai 201318, P.R. China
Published online on: July 13, 2017 https://doi.org/10.3892/mmr.2017.6963
Pages: 3125-3132
Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Shexiang Tongxin Dropping Pill (STP) is an established traditional Chinese medicine that is widely used for the treatment of ischemic heart disease (IHD), although its mechanisms remain unclear. The present study investigated the protective effects of STP following pituitrin (PTT)‑induced myocardial ischemia in rats. ST‑segment elevation, blood rheology, and the serum levels of creatine kinase‑MB (CK‑MB) and lactate dehydrogenase (LDH) were measured. Following heart excision, histological analysis using hematoxylin and eosin and terminal deoxynucleotidyl transferase dUTP nick end labeling were performed. The mRNA expression levels of B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑associated X protein (Bax) were determined using reverse transcription‑quantitative polymerase chain reaction, and their protein expression was detected using immunohistochemistry. The results demonstrated that STP treatment protected against ST elevation, lowered whole blood viscosity, and reduced the serum levels of CK‑MB and LDH following acute myocardial ischemia. In addition, STP treatment restored the histopathological change following PTT‑induced myocardial ischemia, and resulted in downregulated expression of Bax and upregulated expression of Bcl‑2 in myocardial tissue. The present study demonstrates the cardioprotective ability of STP in a rat model of myocardial ischemic injury, which may be attributed to its anti‑apoptotic properties. The cardioprotective properties of STP require further investigation to determine whether it may be used for the clinical treatment of IHDs.

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1	Fan J, Li GQ, Liu J, Wang W, Wang M, Qi Y, Xie WX, Liu J, Zhao F, Li Y and Zhao D: Impact of cardiovascular disease deaths on life expectancy in Chinese population. Biomed Environ Sci. 27:162–168. 2014.PubMed/NCBI
2	Sivaraman V and Yellon DM: Pharmacologic therapy that simulates conditioning for cardiac ischemic/reperfusion injury. J Cardiovasc Pharmacol Ther. 19:83–96. 2014. View Article : Google Scholar : PubMed/NCBI
3	Guo X, Cao W, Yao J, Yuan Y, Hong Y, Wang X and Xing J: Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury. Mol Med Rep. 11:2227–2233. 2015.PubMed/NCBI
4	Panda S, Kar A and Ramamurthy V: Cardioprotective effect of vincristine on isoproterenol-induced myocardial necrosis in rats. Eur J Pharmacol. 723:451–458. 2014. View Article : Google Scholar : PubMed/NCBI
5	Chen D, Lin S, Xu W, Huang M, Chu J, Xiao F, Lin J and Peng J: Qualitative and quantitative analysis of the major constituents in shexiang tongxin dropping pill by HPLC-Q-TOF-MS/MS and UPLC-QqQ-MS/MS. Molecules. 20:18597–18619. 2015. View Article : Google Scholar : PubMed/NCBI
6	Xiong M, Jia C, Cui J, Wang P, Du X, Yang Q, Zhu Y, Wang W, Zhang T and Chen Y: Shexiang Tongxin dropping pill attenuates atherosclerotic lesions in ApoE deficient mouse model. J Ethnopharmacol. 159:84–92. 2015. View Article : Google Scholar : PubMed/NCBI
7	Hua X, Zhan Y and Li Z: Effect of Shexiang Tongxin Dropping Pill on Cardiac Function in Patients with Chronic Heart Failure. Chin J Integr Med Cardio-/Cerebrovasc Dis. 2011.
8	Ning H: The analysis of Shexiang Tongxin dropping pill on treating coronary heart disease angina pectoris curative effect. Chin J Geriatr Care. 2012.
9	Sui W: Clinical observation of Shexiang Tongxin dropping pill in treating senile unstable agina pectoris. Chin Community Doctors. 9:22011.
10	Zhang JXX and Wang W: Shexiang Tongxin dropping pill Evaluate the Efficacy of the Treatment of Unstable Angina. Chin J Integr Med Cardio-/Cerebrovasc Dis. 9:22011.
11	Liu ZH QXD-LYLYL-YJJ-GSA-JXX-GWW-M: The Protective Function of Shexiang on Endothelial Injury Induced by AngiotensinIIOsmotic Pump in the Rat. Chin J Clin Med. 2009.
12	Jiang C: Protective Efect of Qi-dan-hua-tan Decoction on Acute Myocardial Ischemia Injury of Rat. J Chengdu Univ Traditional Chin Med. 2011.
13	Liu X, Liu H, Zeng Z, Zhou W, Liu J and He Z: Pharmacokinetics of ligustrazine ethosome patch in rats and anti-myocardial ischemia and anti-ischemic reperfusion injury effect. Int J Nanomedicine. 6:1391–1398. 2011. View Article : Google Scholar : PubMed/NCBI
14	Zhang M and Cao H: Research on the Animal Model of Coronary Heart Disease Due to Heart Yang Deficiency. China J Basic Med Traditional Chin Med. 2002.
15	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
16	Qian Y, Wang S, Xie Y, Wang J, Li H, Zhou X and Liu W: Effect of salvianolic Acid b and paeonol on blood lipid metabolism and hemorrheology in myocardial ischemia rabbits induced by pituitruin. Int J Mol Sci. 11:3696–3704. 2010. View Article : Google Scholar : PubMed/NCBI
17	Black JW: Electrocardiographic changes produced in rabbits by vasopressin (pitressin) and their alteration by prolonged treatment with a commercial heart extract. J Pharm Pharmacol. 12:87–94. 1960. View Article : Google Scholar : PubMed/NCBI
18	Jr JH and Rikkers LF: Success of medical and surgical management of acute variceal hemorrhage. Am J Surg. 140:816–820. 1980. View Article : Google Scholar : PubMed/NCBI
19	Simpson LO: Angina, ischemic heart disease and blood viscosity. BMJ. 339:464. 2015.
20	Sokolov EI, Zykova AA, Sushchik VV and Goncharov IN: Blood viscosity in patients with ischemic heart disease. Kardiologiia. 54:9–14. 2014.(In Russian). View Article : Google Scholar : PubMed/NCBI
21	Deng C, Sun Z, Tong G, Yi W, Ma L, Zhao B, Cheng L, Zhang J, Cao F and Yi D: α-Lipoic acid reduces infarct size and preserves cardiac function in rat myocardial ischemia/reperfusion injury through activation of PI3K/Akt/Nrf2 pathway. PLoS One. 8:e583712013. View Article : Google Scholar : PubMed/NCBI
22	Gottlieb RA and Engler RL: Apoptosis in myocardial ischemia-reperfusion. Ann N Y Acad Sci. 874:412–426. 1999. View Article : Google Scholar : PubMed/NCBI
23	Veinot JP, Gattinger DA and Fliss H: Early apoptosis in human myocardial infarcts. Hum Pathol. 28:485–892. 1997. View Article : Google Scholar : PubMed/NCBI
24	Shen M, Wu RX, Zhao L, Li J, Guo HT, Fan R, Cui Y, Wang YM, Yue SQ and Pei JM: Resveratrol attenuates ischemia/reperfusion injury in neonatal cardiomyocytes and its underlying mechanism. PLoS One. 7:e512232012. View Article : Google Scholar : PubMed/NCBI
25	Song M, Huang L, Zhao G and Song Y: Beneficial effects of a polysaccharide from Salvia miltiorrhiza on myocardial ischemia-reperfusion injury in rats. Carbohydr Polym. 98:1631–1636. 2013. View Article : Google Scholar : PubMed/NCBI
26	Caroppi P, Sinibaldi F, Fiorucci L and Santucci R: Apoptosis and human diseases: Mitochondrion damage and lethal role of released cytochrome C as proapoptotic protein. Curr Med Chem. 16:4058–4065. 2009. View Article : Google Scholar : PubMed/NCBI
27	Foadoddini M, Esmailidehaj M, Mehrani H, Sadraei SH, Golmanesh L, Wahhabaghai H, Valen G and Khoshbaten A: Pretreatment with hyperoxia reduces in vivo infarct size and cell death by apoptosis with an early and delayed phase of protection. Eur J Cardiothorac Surg. 39:233–240. 2011. View Article : Google Scholar : PubMed/NCBI
28	Wang YL, Wang CY, Zhang BJ and Zhang ZZ: Shenfu injection suppresses apoptosis by regulation of Bcl-2 and caspase-3 during hypoxia/reoxygenation in neonatal rat cardiomyocytes in vitro. Mol Biol Rep. 36:365–370. 2009. View Article : Google Scholar : PubMed/NCBI
29	Xu J, Min Z, Jian O, Wang J, Zhang Q, Xu Y, Xu Y, Zhang Q, Xu X and Zeng H: Gambogic acid induces mitochondria-dependent apoptosis by modulation of Bcl-2 and Bax in mantle cell lymphoma JeKo-1 cells. Chin J Cancer Res. 25:183–191. 2013.PubMed/NCBI
30	Ji L, Fu FL, Liu W, Cai X, Zhang L, Zheng Q, Zhang H and Gao F: Insulin attenuates myocardial ischemia/reperfusion injury via reducing oxidative/nitrative stress. Am J Physiol Endocrinol Metab. 298:E871–E880. 2010. View Article : Google Scholar : PubMed/NCBI
31	Liao YH, Xia N, Zhou SF, Tang TT, Yan XX, Lv BJ, Nie SF, Wang J, Iwakura Y, Xiao H, et al: Interleukin-17A contributes to myocardial ischemia/reperfusion injury by regulating cardiomyocyte apoptosis and neutrophil infiltration. J Am Coll Cardiol. 59:420–429. 2012. View Article : Google Scholar : PubMed/NCBI