Marine collagen peptides reduce endothelial cell injury in diabetic rats by inhibiting apoptosis and the expression of coupling factor 6 and microparticles

Zhu,Cuifeng; Zhang,Wei; Liu,Jianguo; Mu,Bo; Zhang,Fan; Lai,Nannan; Zhou,Jianxin; Xu,Aimin; Li,Yong

doi:10.3892/mmr.2017.7061

Marine collagen peptides reduce endothelial cell injury in diabetic rats by inhibiting apoptosis and the expression of coupling factor 6 and microparticles

Authors:
- Cuifeng Zhu
- Wei Zhang
- Jianguo Liu
- Bo Mu
- Fan Zhang
- Nannan Lai
- Jianxin Zhou
- Aimin Xu
- Yong Li
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Affiliations: Department of Nutrition, Southern Medical University Shenzhen Hospital, Shenzhen, Guangdong 518000, P.R. China, Department of Hematology, Shenzhen Hengsheng Affiliated Hospital, Southern Medical University, Shenzhen, Guangdong 518000, P.R. China, Department of Health Management, Southern Medical University Shenzhen Hospital, Shenzhen, Guangdong 518000, P.R. China, Department of Nutrition, The Second People's Hospital of Yunnan, Kunming, Yunnan 650000, P.R. China, Department of Endocrinology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China, Institute of Basic Medicine Shandong Academy of Medical Sciences, Jinan, Shandong 250000, P.R. China, Department of Endocrinology, Shenzhen Hengsheng Affiliated Hospital, Southern Medical University, Shenzhen, Guangdong 518000, P.R. China, Department of Medicine, Li Kashing Faculty of Medicine, The State Key Laboratory of Pharmaceutical Biotechnology, University of Hong Kong, Hong Kong 325035, P.R. China, Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, P.R. China
Published online on: July 21, 2017 https://doi.org/10.3892/mmr.2017.7061
Pages: 3947-3957
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to elucidate the role of marine collagen peptides (MCPs) in protection of carotid artery vascular endothelial cells (CAVECs) in type 2 diabetes mellitus (T2DM), and the mechanism underlying this process. In an in vivo experiment, diabetic Wistar rats were divided randomly into four groups (n=10/group): Diabetes control, and three diabetes groups administered low, medium and high doses of MCPs (2.25, 4.5 and 9.0 g/kg body weight/day, respectively). Another 10 healthy rats served as the control. In an in vitro experiment, human umbilical‑vein endothelial cells (HUVECs) were incubated in normal and high concentrations of glucose with or without MCPs (3.0, 15.0 and 30.0 mg/ml, respectively) for 24, 48 or 72 h. Blood vessel/endothelial construction, inflammatory exudation and associated molecular biomarkers in CAVECs were detected and analyzed. The results of the present study demonstrated that in rats, MCP treatment for 4 weeks significantly lowered blood glucose and attenuated endothelial thinning and inflammatory exudation in carotid‑artery vascular endothelial cells. In vitro, the high‑glucose intervention significantly increased cell apoptosis in HUVECs, and medium and high doses of MCPs (4.5 and 9.0 g/kg body weight/day, respectively) partially ameliorated this high glucose‑mediated apoptosis and decreased levels of apoptosis biomarkers. In conclusion, a moderate oral MCP dose (≥4.5 g/kg body weight/day) may be a novel therapeutic tool to protect against early cardiovascular complications associated with T2DM by inhibiting apoptosis and reducing the expression of coupling factor 6 and microparticles.