E‑cadherin involvement in human lens epithelial cell transdifferentiation may be associated with N‑cadherin

  • Authors:
    • Lei Huang
    • Jie Jiang
    • Qiang Guo
    • Hongbin Yang
  • View Affiliations

  • Published online on: August 2, 2017     https://doi.org/10.3892/mmr.2017.7132
  • Pages: 5031-5035
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Abstract

E-cadherin, β-catenin and N‑cadherin serve key roles in the epithelial‑to‑mesenchymal transition (EMT) that leads to human lens epithelial cell (LEC) transdifferentiation and subsequent cataract formation. The present study aimed to investigate the role of E‑cadherin in LEC transdifferentiation. SRA01/04 human LECs were transfected with E‑cadherin short interfering (si)RNA (E‑cadherin siRNA group), negative control siRNA (NC group) or the transfection regent Lipofectamine 2000 (blank group). Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect mRNA expression levels of E‑cadherin, N‑cadherin and β‑catenin, and western blot analysis was performed to measure the protein expression levels in the three groups. SRA01/04 cells transfected with E‑cadherin siRNA exhibited a significant decrease in the mRNA expression level of E‑cadherin (P<0.05) and N‑cadherin (P<0.05), whereas no significant changes were identified for β‑catenin expression (P>0.05). Consistent with the results of RT‑qPCR, western blotting demonstrated that the protein expression levels of E‑cadherin and N‑cadherin were notably decreased in E‑cadherin siRNA‑transfected cells, whereas the protein expression level of β‑catenin remained unchanged. Results from the present study indicated that E‑cadherin may be involved in human LEC transdifferentiation by affecting N‑cadherin expression.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Huang L, Jiang J, Guo Q and Yang H: E‑cadherin involvement in human lens epithelial cell transdifferentiation may be associated with N‑cadherin. Mol Med Rep 16: 5031-5035, 2017
APA
Huang, L., Jiang, J., Guo, Q., & Yang, H. (2017). E‑cadherin involvement in human lens epithelial cell transdifferentiation may be associated with N‑cadherin. Molecular Medicine Reports, 16, 5031-5035. https://doi.org/10.3892/mmr.2017.7132
MLA
Huang, L., Jiang, J., Guo, Q., Yang, H."E‑cadherin involvement in human lens epithelial cell transdifferentiation may be associated with N‑cadherin". Molecular Medicine Reports 16.4 (2017): 5031-5035.
Chicago
Huang, L., Jiang, J., Guo, Q., Yang, H."E‑cadherin involvement in human lens epithelial cell transdifferentiation may be associated with N‑cadherin". Molecular Medicine Reports 16, no. 4 (2017): 5031-5035. https://doi.org/10.3892/mmr.2017.7132