Knockdown of lncRNA HNF1A-AS1 inhibits oncogenic phenotypes in colorectal carcinoma
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- Published online on: August 4, 2017 https://doi.org/10.3892/mmr.2017.7175
- Pages: 4694-4700
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Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
Long non-coding RNAs (lncRNAs) have been demonstrated to serve important roles in the development and progression of cancer. Recently HNF1A antisense RNA 1 (HNF1A‑AS1), a lncRNA, has been reported as exhibiting a potential oncogenic role in the development of many types of cancer. However, the expression and the role of HNF1A‑AS1 in colorectal carcinoma (CRC) remains unclear. In the present study, the role of HNF1A‑AS1 in CRC was examined for the first time and its correlation with CRC cell biological behaviors was analyzed. The results demonstrated that HNF1A‑AS1 was distinctly upregulated in CRC tissues and associated with CRC metastasis to the lymph nodes. Reverse transcription‑quantitative polymerase chain reaction revealed that HNF1A‑AS1 was also upregulated in CRC cell lines and localized in the nucleus. In addition, knockdown of HNF1A‑AS1 expression notably inhibited CRC cell proliferation, migration, invasion and colony formation, and suppressed S‑phase entry in vitro. Taken together, these results suggested that HNF1A‑AS1 might serve as a promising prognostic marker for CRC tumorigenesis and progression.
