Open Access

Naringenin attenuates fibroblast activation and inflammatory response in a mechanical stretch-induced hypertrophic scar mouse model

  • Authors:
    • Shengzhou Shan
    • Yifan Zhang
    • Min Wu
    • Bo Yi
    • Jing Wang
    • Qingfeng Li
  • View Affiliations

  • Published online on: August 10, 2017     https://doi.org/10.3892/mmr.2017.7209
  • Pages: 4643-4649
  • Copyright: © Shan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The pathogenesis and therapy of hypertrophic scars (HS) have not yet been established. The aim of the present study was to investigate the potential effect of naringenin on HS and its underlying mechanisms. The mouse model of HS was prepared by a mechanical stretch device and then treated with naringenin at various concentrations. Histological studies were performed to evaluate scar hypertrophy by hematoxylin and eosin, as well as Masson's trichrome staining. The activation of HS fibroblasts was determined based on reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), western blotting and immunohistochemical staining. Following observing the retention of inflammation cells by immunohistochemistry, the cytokines, including tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, IL‑6 and transforming growth factor (TGF)‑β1, mRNA and protein levels were quantitated by RT‑qPCR, ELISA and western blotting methods. As a result, naringenin significantly inhibited the formation of HS in a concentration‑dependent manner. In addition, naringenin inhibited fibroblast activation and inflammatory cell recruitment. In addition, mRNA and protein expression levels of TNF‑α, IL‑1β, IL‑6 and TGF‑β1 were downregulated following naringenin treatment. The current study highlighted a new pharmacological activity of naringenin on HS. The mechanism of action of naringenin was associated with the inhibition of fibroblast activation and local inflammation. These results suggested that naringenin may serve as a novel agent for treatment of HS.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Shan S, Zhang Y, Wu M, Yi B, Wang J and Li Q: Naringenin attenuates fibroblast activation and inflammatory response in a mechanical stretch-induced hypertrophic scar mouse model. Mol Med Rep 16: 4643-4649, 2017
APA
Shan, S., Zhang, Y., Wu, M., Yi, B., Wang, J., & Li, Q. (2017). Naringenin attenuates fibroblast activation and inflammatory response in a mechanical stretch-induced hypertrophic scar mouse model. Molecular Medicine Reports, 16, 4643-4649. https://doi.org/10.3892/mmr.2017.7209
MLA
Shan, S., Zhang, Y., Wu, M., Yi, B., Wang, J., Li, Q."Naringenin attenuates fibroblast activation and inflammatory response in a mechanical stretch-induced hypertrophic scar mouse model". Molecular Medicine Reports 16.4 (2017): 4643-4649.
Chicago
Shan, S., Zhang, Y., Wu, M., Yi, B., Wang, J., Li, Q."Naringenin attenuates fibroblast activation and inflammatory response in a mechanical stretch-induced hypertrophic scar mouse model". Molecular Medicine Reports 16, no. 4 (2017): 4643-4649. https://doi.org/10.3892/mmr.2017.7209