Open Access

High glucose and high insulin conditions promote MCF‑7 cell proliferation and invasion by upregulating IRS1 and activating the Ras/Raf/ERK pathway

Corrigendum in: /10.3892/mmr.2023.12967

  • Authors:
    • Mei‑Lin Wei
    • Peng Duan
    • Zhi‑Ming Wang
    • Miao Ding
    • Ping Tu
  • View Affiliations

  • Published online on: August 31, 2017     https://doi.org/10.3892/mmr.2017.7420
  • Pages: 6690-6696
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Diabetes mellitus is associated with an increased risk of breast cancer, but the molecular mechanism underlying this association remains unclear. The aim of the present study was to investigate the effect of high glucose and high insulin conditions on MCF‑7 breast cancer cells and to elucidate the molecular mechanisms underlying these effects. High glucose and high insulin conditions resulted in increased viability, proliferation, and invasion in MCF‑7 cells compared with normal glucose and low insulin conditions. Reverse transcription‑quantitative polymerase chain reaction and western blot analyses revealed that insulin receptor substrate 1 (IRS1) was significantly upregulated following high glucose and high insulin treatment compared with normal glucose and low insulin conditions. Furthermore, high glucose and high insulin treatment increased the Ras family of proto‑oncogenes (Ras) and RAF1 proto‑oncogene (Raf‑1) protein expression, and activated the phosphorylation of extracellular signal‑regulated kinase (ERK) 1/2. These findings suggest that high glucose and high insulin conditions promoted the proliferation and invasion of MCF‑7 cells by upregulating IRS1 and activating the Ras/Raf/ERK pathway.
View Figures
View References

Related Articles

Journal Cover

November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wei ML, Duan P, Wang ZM, Ding M and Tu P: High glucose and high insulin conditions promote MCF‑7 cell proliferation and invasion by upregulating IRS1 and activating the Ras/Raf/ERK pathway Corrigendum in /10.3892/mmr.2023.12967. Mol Med Rep 16: 6690-6696, 2017.
APA
Wei, M., Duan, P., Wang, Z., Ding, M., & Tu, P. (2017). High glucose and high insulin conditions promote MCF‑7 cell proliferation and invasion by upregulating IRS1 and activating the Ras/Raf/ERK pathway Corrigendum in /10.3892/mmr.2023.12967. Molecular Medicine Reports, 16, 6690-6696. https://doi.org/10.3892/mmr.2017.7420
MLA
Wei, M., Duan, P., Wang, Z., Ding, M., Tu, P."High glucose and high insulin conditions promote MCF‑7 cell proliferation and invasion by upregulating IRS1 and activating the Ras/Raf/ERK pathway Corrigendum in /10.3892/mmr.2023.12967". Molecular Medicine Reports 16.5 (2017): 6690-6696.
Chicago
Wei, M., Duan, P., Wang, Z., Ding, M., Tu, P."High glucose and high insulin conditions promote MCF‑7 cell proliferation and invasion by upregulating IRS1 and activating the Ras/Raf/ERK pathway Corrigendum in /10.3892/mmr.2023.12967". Molecular Medicine Reports 16, no. 5 (2017): 6690-6696. https://doi.org/10.3892/mmr.2017.7420