Open Access

Differential miRNA expression profiles in human keratinocytes in response to protein kinase C inhibitor

  • Authors:
    • Yang Liu
    • Liangchen Zhong
    • Dewu Liu
    • Hua Ye
    • Yuangui Mao
    • Yanghong Hu
  • View Affiliations

  • Published online on: September 8, 2017     https://doi.org/10.3892/mmr.2017.7447
  • Pages: 6608-6619
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Aberrant expression of microRNAs (miRNAs) is widely accepted to be involved in keratinocyte differentiation and to be dependent on activation of the protein kinase C (PKC) pathway. However, the miRNA profiles and biological characteristics of keratinocytes induced by specific inhibitors of PKC have yet to be elucidated. The present study aimed to explore the differential miRNA expression profiles in keratinocytes treated with the PKC inhibitor GF109203X, by conducting a bioinformatics analysis. Parts of the GF109203X‑induced keratinocytes formed distinct clones after 2 days of culture, and the expression of intergrin β1, cytokeratin (CK)19 and CK14 were positive, whereas CK10 expression was negative. A total of 79 miRNAs were differentially expressed in keratinocytes treated with GF109203X, among which 45 miRNAs were upregulated and 34 were downregulated. The significantly upregulated microRNAs includedhsa‑miR‑1‑3p and miR‑181c‑5p, whereas hsa‑miR‑31‑5p and hsa‑let‑7c‑3p were significantly downregulated. In addition, the results of reverse transcription‑quantitative polymerase chain reaction exhibited consistency with the microarray results. An enrichment analysis demonstrated that certain target genes of the differentially expressed miRNAs serve an important role in cell proliferation and differentiation, cell cycle progression and apoptosis, etc. These results revealed that GF109203X induced the differential expression of certain miRNAs when keratinocytes began showing the characteristics of epidermal‑like stem cells, which may provide a novel approach for wound healing and regeneration of skin tissues.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Liu Y, Zhong L, Liu D, Ye H, Mao Y and Hu Y: Differential miRNA expression profiles in human keratinocytes in response to protein kinase C inhibitor. Mol Med Rep 16: 6608-6619, 2017.
APA
Liu, Y., Zhong, L., Liu, D., Ye, H., Mao, Y., & Hu, Y. (2017). Differential miRNA expression profiles in human keratinocytes in response to protein kinase C inhibitor. Molecular Medicine Reports, 16, 6608-6619. https://doi.org/10.3892/mmr.2017.7447
MLA
Liu, Y., Zhong, L., Liu, D., Ye, H., Mao, Y., Hu, Y."Differential miRNA expression profiles in human keratinocytes in response to protein kinase C inhibitor". Molecular Medicine Reports 16.5 (2017): 6608-6619.
Chicago
Liu, Y., Zhong, L., Liu, D., Ye, H., Mao, Y., Hu, Y."Differential miRNA expression profiles in human keratinocytes in response to protein kinase C inhibitor". Molecular Medicine Reports 16, no. 5 (2017): 6608-6619. https://doi.org/10.3892/mmr.2017.7447