Exposure of keratinocytes to non‑thermal dielectric barrier discharge plasma increases the level of 8‑oxoguanine via inhibition of its repair enzyme

Kim,Ki Cheon; Ruwan Kumara,Madduma Hewage Susara; Kang,Kyoung Ah; Piao,Mei Jing; Oh,Min Chang; Ryu,Yea Seong; Jo,Jin Oh; Mok,Young Sun; Shin,Jennifer H.; Park,Yeunsoo; Kim,Seong Bong; Yoo,Suk Jae; Hyun,Jin Won

doi:10.3892/mmr.2017.7454

Exposure of keratinocytes to non‑thermal dielectric barrier discharge plasma increases the level of 8‑oxoguanine via inhibition of its repair enzyme

Authors:
- Ki Cheon Kim
- Madduma Hewage Susara Ruwan Kumara
- Kyoung Ah Kang
- Mei Jing Piao
- Min Chang Oh
- Yea Seong Ryu
- Jin Oh Jo
- Young Sun Mok
- Jennifer H. Shin
- Yeunsoo Park
- Seong Bong Kim
- Suk Jae Yoo
- Jin Won Hyun
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Affiliations: Department of Biochemistry, School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea, Department of Chemical and Biological Engineering, Jeju National University, Jeju 63243, Republic of Korea, Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea, Plasma Technology Research Center, National Fusion Research Institute, Gunsan 54004, Republic of Korea
Published online on: September 11, 2017 https://doi.org/10.3892/mmr.2017.7454
Pages: 6870-6875

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Abstract

Oxidative stress enhances cellular DNA oxidation and may cause mutations in DNA bases, including 8‑oxoguanine (8‑oxoG). Our recent study reported that exposure of cells to non‑thermal dielectric barrier discharge (DBD) plasma generates reactive oxygen species and damages DNA. The present study investigated the effect of non‑thermal DBD plasma exposure on the formation of 8‑oxoG in HaCaT human keratinocytes. Cells exposed to DBD plasma exhibited increased level of 8‑oxoG. In addition, mRNA and protein expression levels of 8‑oxoguanine glycosylase 1 (OGG1), an 8‑oxoG repair enzyme, were reduced in plasma‑exposed cells. Furthermore, the expression level of nuclear factor erythroid 2‑related factor 2 (Nrf2), a transcription factor that regulates OGG1 gene expression, was reduced following exposure to DBD plasma. Pretreatment of cells with an antioxidant, N‑acetyl cysteine (NAC), prior to plasma exposure suppressed the formation of 8‑oxoG and restored the expression levels of OGG1 and Nrf2. In addition, phosphorylation of protein kinase B (Akt), which regulates the activation of Nrf2, was reduced following plasma exposure. However, phosphorylation was restored by pretreatment with NAC. These findings suggested that non‑thermal DBD plasma exposure generates 8‑oxoG via inhibition of the Akt‑Nrf2‑OGG1 signaling pathway in HaCaT cells.

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