Sinulariolide suppresses LPS‑induced phenotypic and functional maturation of dendritic cells

Chung,Ting‑Wen; Li,Yi‑Rong; Huang,Wei Yuan; Su,Jui‑Hsin; Chan,Hong‑Lin; Lin,Sheng‑Hao; Liu,Chin‑San; Lin,Shih‑Chao; Lin,Chi‑Chien; Lin,Ching‑Hsiung

doi:10.3892/mmr.2017.7480

Sinulariolide suppresses LPS‑induced phenotypic and functional maturation of dendritic cells

Authors:
- Ting‑Wen Chung
- Yi‑Rong Li
- Wei Yuan Huang
- Jui‑Hsin Su
- Hong‑Lin Chan
- Sheng‑Hao Lin
- Chin‑San Liu
- Shih‑Chao Lin
- Chi‑Chien Lin
- Ching‑Hsiung Lin
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Affiliations: Institute of Bioinformatics and Structural Biology and Department of Medical Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan, R.O.C., Department of Medical Research, Changhua Christian Hospital, Changhua 50006, Taiwan, R.O.C., Institute of Biomedical Science, National Chung‑Hsing University, Taichung 40227, Taiwan, R.O.C., National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan, R.O.C., Institute of Genomics and Bioinformatics, National Chung‑Hsing University, Taichung 40227, Taiwan, R.O.C.
Published online on: September 13, 2017 https://doi.org/10.3892/mmr.2017.7480
Pages: 6992-7000

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Abstract

The dendritic cell (DC) maturation process is essential for the development of T cell responses and immune tolerance. Accordingly, DCs are considered a major target in the development of immunomodulating agents. In the present study, the effect of sinulariolide, an active compound isolated from the cultured soft coral Sinularia flexibilis, on lipopolysaccharide (LPS)‑induced murine bone marrow‑derived DCs was evaluated. The different phenotypes, cytokine secretion and the mix‑lymphocyte reaction of DCs were detected using flow cytometry and ELISA. The experimental results revealed that the phenotypic and functional maturation of DCs stimulated by LPS were markedly reduced by sinulariolide in a concentration‑dependent manner, including the expression of co‑stimulatory molecules (CD40, CD80 and CD86). In addition, sinulariolide reduced the release of tumor necrosis factor‑α, interleukin (IL)‑6, IL‑12 and nitric oxide from the LPS‑activated DCs, decreased their abilities to stimulate allogeneic T cell proliferation, and inhibited LPS‑induced nuclear factor‑κB pathways. These findings offer novel insight into the immunopharmacological function of sinulariolide and its effects on DCs.

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