GOLPH3 promotes cell proliferation and tumorigenicity in esophageal squamous cell carcinoma via mTOR and Wnt/β‑catenin signal activation

Wang,Jian‑Hua; Yuan,Lin‑Jing; Liang,Rong‑Xin; Liu,Zi‑Gang; Li,Bo‑Hai; Wen,Zhe‑Sheng; Huang,Shu‑Ting; Zheng,Min

doi:10.3892/mmr.2017.7495

GOLPH3 promotes cell proliferation and tumorigenicity in esophageal squamous cell carcinoma via mTOR and Wnt/β‑catenin signal activation

Authors:
- Jian‑Hua Wang
- Lin‑Jing Yuan
- Rong‑Xin Liang
- Zi‑Gang Liu
- Bo‑Hai Li
- Zhe‑Sheng Wen
- Shu‑Ting Huang
- Min Zheng
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Affiliations: Department of Cardiovascular Surgery, Guangdong No. 2 Provincial People's Hospital, Guangzhou, Guangdong 510317, P.R. China, Department of Gynecology, The First Affiliated Hospital of Sun Yat‑Sen University Guangzhou, Guangdong 510080, P.R. China, Department of Chest, Guangdong No. 2 Provincial People's Hospital, Guangzhou, Guangdong 510317, P.R. China, Department of Chest, Sun Yat‑Sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China, Department of Gynecology, Sun Yat‑Sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China
Published online on: September 13, 2017 https://doi.org/10.3892/mmr.2017.7495
Pages: 7138-7144

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Abstract

The authors' previous study demonstrated that Golgi phosphoprotein 3 (GOLPH3) was significantly overexpressed in esophageal squamous cell carcinoma (ESCC), correlating with poor patient survival. In the present study, GOLPH3 stable overexpression and knockdown KYSE‑140 cell lines were constructed. Cell proliferation, colony formation, cell cycle progression and tumorigenesis assays were performed. The results revealed that GOLPH3 promoted ESCC cell growth and proliferation. The effects of GOLPH3 on the mechanistic target of rapamycin (mTOR) and Wnt/β‑catenin signaling pathways were investigated using western blot analyis and dual‑luciferase reporter assays, and were observed to be activated in cells with GOLPH3 overexpression. Furthermore, overexpression of GOLPH3 resulted in the downregulation of p21 protein, upregulation of cyclin D1 and increased retinoblastoma‑associated protein phosphorylation, consequently leading to accelerated cell cycle progression. In addition, GOLPH3 knockdown resulted in reversed effects. The results of the current study suggest that GOLPH3 serves an important role in promoting tumorigenicity of ESCC via mTOR and Wnt/β‑catenin signaling pathway activation.

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