Open Access

Transplantation of periodontal ligament cell sheets expressing human β‑defensin‑3 promotes anti‑inflammation in a canine model of periodontitis

  • Authors:
    • Minwen Zhu
    • Bo Miao
    • Jianhua Zhu
    • Haiyan Wang
    • Zengtong Zhou
  • View Affiliations

  • Published online on: September 19, 2017     https://doi.org/10.3892/mmr.2017.7514
  • Pages: 7459-7467
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Periodontitis is a chronic oral inflammatory disease caused by microorganisms. Human β‑defensin‑3 (HBD‑3) is an endogenous antimicrobial peptide that inhibits a broad spectrum of microorganisms. Cell sheet technology has been widely applied in tissue and organ reconstructions. In the current study, it was aimed to investigate the anti‑inflammatory effect of periodontal tissue engineered by HBD‑3 gene‑modified periodontal ligament cell (PDLC) sheets, and to identify a suitable method of promoting the regeneration of periodontal tissues. Western blot analysis and antimicrobial tests were used to confirm the expression of HBD‑3. The effect of the cell sheets on anti‑inflammatory activity and bone remodeling in a dog model of periodontitis was demonstrated by immunohistochemistry. The results demonstrated that the transfected PDLCs stably expressed HBD‑3. Periodontal pathogens were susceptible to the antimicrobial activity of the cell sheets. In addition, the cell sheets relieved the bone resorption caused by inflammation in the in vivo model. HBD‑3 may potentially be applied in the treatment of periodontitis and may function as osteogenic promoter via its anti‑inflammatory effect.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhu M, Miao B, Zhu J, Wang H and Zhou Z: Transplantation of periodontal ligament cell sheets expressing human β‑defensin‑3 promotes anti‑inflammation in a canine model of periodontitis. Mol Med Rep 16: 7459-7467, 2017.
APA
Zhu, M., Miao, B., Zhu, J., Wang, H., & Zhou, Z. (2017). Transplantation of periodontal ligament cell sheets expressing human β‑defensin‑3 promotes anti‑inflammation in a canine model of periodontitis. Molecular Medicine Reports, 16, 7459-7467. https://doi.org/10.3892/mmr.2017.7514
MLA
Zhu, M., Miao, B., Zhu, J., Wang, H., Zhou, Z."Transplantation of periodontal ligament cell sheets expressing human β‑defensin‑3 promotes anti‑inflammation in a canine model of periodontitis". Molecular Medicine Reports 16.5 (2017): 7459-7467.
Chicago
Zhu, M., Miao, B., Zhu, J., Wang, H., Zhou, Z."Transplantation of periodontal ligament cell sheets expressing human β‑defensin‑3 promotes anti‑inflammation in a canine model of periodontitis". Molecular Medicine Reports 16, no. 5 (2017): 7459-7467. https://doi.org/10.3892/mmr.2017.7514