Open Access

Asymmetric dimethylarginine downregulates sarco/endoplasmic reticulum calcium‑ATPase 3 and induces endoplasmic reticulum stress in human umbilical vein endothelial cells

  • Authors:
    • Weikang Guo
    • Zongli Diao
    • Wenhu Liu
  • View Affiliations

  • Published online on: September 19, 2017     https://doi.org/10.3892/mmr.2017.7529
  • Pages: 7541-7547
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cardiovascular disease is the leading cause of mortality in patients with chronic kidney disease. Endothelial cell injury and apoptosis may promote atherosclerosis and cardiovascular disease. The present study investigated the potential mechanisms of asymmetric dimethylarginine (ADMA)‑induced apoptosis in human umbilical vein endothelial cells (HUVECs). It was demonstrated that ADMA decreased B‑cell lymphoma‑2 expression and increased cleaved‑caspase‑3 expression. Furthermore, terminal deoxynucleotidyl transferase (TdT)‑mediated‑digoxigenin‑11‑dUTP nick end labeling results indicated that ADMA induced apoptosis in HUVECs. These results suggest a potential mechanism of ADMA‑induced endothelial cell injury. It was also verified that ADMA induced the expression of phosphorylated protein kinase RNA‑like ER kinase, inositol requiring enzyme‑1, C/EBP homologous protein and glucose‑regulated protein, indicating activation of the endoplasmic reticulum (ER) stress response. Impaired function of sarco/endoplasmic reticulum calcium‑ATPase (SERCA) is considered a major contributor to ER stress. It was demonstrated that ADMA induced a significant downregulation of SERCA3, however not SERCA2b. Overall, the results indicated that ADMA induced apoptosis in HUVECs, and that this effect was closely associated with induction of ER stress and decreased SERCA3 expression.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Guo W, Diao Z and Liu W: Asymmetric dimethylarginine downregulates sarco/endoplasmic reticulum calcium‑ATPase 3 and induces endoplasmic reticulum stress in human umbilical vein endothelial cells. Mol Med Rep 16: 7541-7547, 2017.
APA
Guo, W., Diao, Z., & Liu, W. (2017). Asymmetric dimethylarginine downregulates sarco/endoplasmic reticulum calcium‑ATPase 3 and induces endoplasmic reticulum stress in human umbilical vein endothelial cells. Molecular Medicine Reports, 16, 7541-7547. https://doi.org/10.3892/mmr.2017.7529
MLA
Guo, W., Diao, Z., Liu, W."Asymmetric dimethylarginine downregulates sarco/endoplasmic reticulum calcium‑ATPase 3 and induces endoplasmic reticulum stress in human umbilical vein endothelial cells". Molecular Medicine Reports 16.5 (2017): 7541-7547.
Chicago
Guo, W., Diao, Z., Liu, W."Asymmetric dimethylarginine downregulates sarco/endoplasmic reticulum calcium‑ATPase 3 and induces endoplasmic reticulum stress in human umbilical vein endothelial cells". Molecular Medicine Reports 16, no. 5 (2017): 7541-7547. https://doi.org/10.3892/mmr.2017.7529