MicroRNA‑340 inhibits tumor cell proliferation, migration and invasion, and induces apoptosis in hepatocellular carcinoma

Wang,Ziyao; Song,Dan; Huang,Ping

doi:10.3892/mmr.2017.7583

MicroRNA‑340 inhibits tumor cell proliferation, migration and invasion, and induces apoptosis in hepatocellular carcinoma

Authors:
- Ziyao Wang
- Dan Song
- Ping Huang
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Affiliations: National Key Clinical Department, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing 400000, P.R. China
Published online on: September 21, 2017 https://doi.org/10.3892/mmr.2017.7583
Pages: 7649-7656

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Abstract

MicroRNAs (miRs) are short RNAs that serve a role in the origination and progression of hepatocellular carcinoma (HCC). miR‑340 has been identified to be a novel tumor suppressor. The present study investigated the antitumor function of miR‑340 in HCC. In the present study, it was detected that miR‑340 was significantly decreased in HCC cancer tissues and human HCC cell lines using reverse transcription‑quantitative polymerase chain reaction analysis. Cell Counting kit‑8 and apoptosis assays demonstrated that miR‑340 reduced cell proliferation and induced cellular apoptosis in HCC cell lines. A Transwell invasion assay demonstrated that miR‑340 suppressed the migration and invasion of HCC cell lines. In addition, S‑phase kinase‑associated protein 2 (SKP2), which may be repressed by miR‑340 in HCC cell lines, was identified to be a potential target of miR‑340. The results of the present study revealed that miR‑340 serves a tumor suppressor role by influencing the proliferation, apoptosis, migration and invasion of HCC cell lines, which may be explained by the downregulation of SKP2 by miR‑340.

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