Whole‑genome scale identification of methylation markers specific for cerebral palsy in monozygotic discordant twins

Jiao,Zhe; Jiang,Zhimei; Wang,Jingtao; Xu,Hui; Zhang,Qiang; Liu,Shuang; Du,Ning; Zhang,Yuanyuan; Qiu,Hongbin

doi:10.3892/mmr.2017.7800

Whole‑genome scale identification of methylation markers specific for cerebral palsy in monozygotic discordant twins

Authors:
- Zhe Jiao
- Zhimei Jiang
- Jingtao Wang
- Hui Xu
- Qiang Zhang
- Shuang Liu
- Ning Du
- Yuanyuan Zhang
- Hongbin Qiu
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Affiliations: Department of Epidemiology, School of Public Health, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Heilongjiang Cerebral Palsy Treatment and Management Center, Jiamusi University, Jiamusi, Heilongjiang 154007, P.R. China, School of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang 154007, P.R. China, School of Public Health, Jiamusi University, Jiamusi, Heilongjiang 154007, P.R. China
Published online on: October 17, 2017 https://doi.org/10.3892/mmr.2017.7800
Pages: 9423-9430
Copyright: © Jiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cerebral palsy (CP) is a severe type of brain disease affecting movement and posture. Although CP has strong genetic and environmental components, considerable differences in the methylome between monozygotic (MZ) twins discordant for CP implicates epigenetic contributors as well. In order to determine the differences in methylation in patients with CP without interference of the interindividual genomic variation, four pairs of MZ twins discordant for CP were profiled for DNA methylation changes using reduced representation bisulfite sequencing on the genomic‑scale. Similar DNA methylation patterns were observed in all samples. However, MZ twins demonstrated higher correlations and closer evolutionary associations compared with the other samples, indicating a stable methylome of MZ twins. A total of 190 differentially methylated genes (DMGs) were identified using Student's t‑test, of which 37 genes were hypermethylated in the CP group while the remainders were hypomethylated compared with control group. The identified DMGs were enriched in several cerebral abnormalities, including cerebral cortical atrophy and cerebral atrophy, suggesting that the occurrence of CP may be associated with the methylation alterations. The neighboring genes of DMGs in the protein‑protein interaction network were enriched in numerous important functions in essential processes. The results of the present study identified important genes that may epigenetically contribute to the occurrence and development of CP in MZ twins, suggesting that the different prevalence of CP in identical twins may be associated with DNA methylation alterations.

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