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miR-320a modulates cell growth and chemosensitivity via regulating ADAM10 in gastric cancer

  • Authors:
    • Xiancai Ge
    • Haiyan Cui
    • Yanbing Zhou
    • Deying Yin
    • Yuanyuan Feng
    • Qun Xin
    • Xianhui Xu
    • Weijing Liu
    • Shanglong Liu
    • Qin Zhang

  • View Affiliations

  • Published online on: October 19, 2017     https://doi.org/10.3892/mmr.2017.7819
  • Pages: 9664-9670
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

MicroRNAs (miRNAs) may function as tumor suppressor or onco‑miRNAs and have critical roles in the pathogenesis of gastric cancer (GC). The exact function and mechanism of miRNA (miR)‑320a in GC remains to be elucidated. The present study performed gain‑ and loss‑of‑function analyses by transfecting cells with mimics or inhibitors and subsequently performing colony formation, proliferation and cisplatin‑sensitivity assays. Additionally, in vivo xenograft models were also performed. Bioinformatics algorithms, luciferase reporter activity assay and western blotting were used to predict the potential target of miR‑320a. Additionally, the effect of knockdown or overexpression of ADAM metallopeptidase domain 10 (ADAM10) on cell growth and chemosensitivity was examined. The expression of miR‑320a and ADAM10 was also determined in primary tumors. The present study revealed that the expression of miR‑320a was reduced in GC cells and ectopic miR‑320a expression significantly inhibited cell growth in vitro and in vivo and enhanced the sensitivity of GC cells to cisplatin. ADAM10 was a direct target of miR‑320a in GC. Knockdown of ADAM10 attenuated the proliferative ability of GC cells, and increased the sensitivity of GC cells to cisplatin. The upregulated ADAM10 accelerated cell growth rate and reduced the cisplatin‑sensitivity of cells. Clinically, a significantly negative correlation was identified between the expression of miR‑320a and mRNA levels of ADAM10 in tumors. The findings of the present study suggested that miR‑320a may function as a tumor suppressor in GC progression and potential therapeutic strategies for GC may be based on the miR‑320a/ADAM10 axis.
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December-2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
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  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Ge X, Cui H, Zhou Y, Yin D, Feng Y, Xin Q, Xu X, Liu W, Liu S, Zhang Q, Zhang Q, et al: miR-320a modulates cell growth and chemosensitivity via regulating ADAM10 in gastric cancer. Mol Med Rep 16: 9664-9670, 2017.
APA
Ge, X., Cui, H., Zhou, Y., Yin, D., Feng, Y., Xin, Q. ... Zhang, Q. (2017). miR-320a modulates cell growth and chemosensitivity via regulating ADAM10 in gastric cancer. Molecular Medicine Reports, 16, 9664-9670. https://doi.org/10.3892/mmr.2017.7819
MLA
Ge, X., Cui, H., Zhou, Y., Yin, D., Feng, Y., Xin, Q., Xu, X., Liu, W., Liu, S., Zhang, Q."miR-320a modulates cell growth and chemosensitivity via regulating ADAM10 in gastric cancer". Molecular Medicine Reports 16.6 (2017): 9664-9670.
Chicago
Ge, X., Cui, H., Zhou, Y., Yin, D., Feng, Y., Xin, Q., Xu, X., Liu, W., Liu, S., Zhang, Q."miR-320a modulates cell growth and chemosensitivity via regulating ADAM10 in gastric cancer". Molecular Medicine Reports 16, no. 6 (2017): 9664-9670. https://doi.org/10.3892/mmr.2017.7819