Naringin protects myocardial cells from doxorubicin‑induced apoptosis partially by inhibiting the p38MAPK pathway

Jian,Chun‑Yan; Ouyang,Han‑Bin; Xiang,Xian‑Hong; Chen,Jia‑Li; Li,Yong‑Xin; Zhou,Xin; Wang,Jin‑Yang; Yang,Yang; Zhong,En‑Yi; Huang,Wen‑Hua; Zhang,Hong‑Wu

doi:10.3892/mmr.2017.7823

Naringin protects myocardial cells from doxorubicin‑induced apoptosis partially by inhibiting the p38MAPK pathway

Authors:
- Chun‑Yan Jian
- Han‑Bin Ouyang
- Xian‑Hong Xiang
- Jia‑Li Chen
- Yong‑Xin Li
- Xin Zhou
- Jin‑Yang Wang
- Yang Yang
- En‑Yi Zhong
- Wen‑Hua Huang
- Hong‑Wu Zhang
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Affiliations: Department of Cardiology, Central People's Hospital of Zhanjiang, Zhanjiang, Guangdong 524000, P.R. China, Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Interventional Radiology, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China
Published online on: October 19, 2017 https://doi.org/10.3892/mmr.2017.7823
Pages: 9457-9463
Copyright: © Jian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Doxorubicin (DOX) has been widely used to treat cancers as a first‑line antitumor drug. However, it causes severe, irreversible, dose‑dependent cardiotoxicity. To evaluate the protective effects of naringin (NRG) on cardiotoxicity, the authors investigated the molecular mechanism of the p38MAPK signaling pathway. H9c2 cells were treated for 24 h by using 5 µmol/l DOX without or with being pretreated by 1 µM NRG for 150 min or by 3 µM SB203580 for 60 min. Cell viability was detected by cell counting kit‑8 assay. Intracellular reactive oxygen species (ROS) levels were detected based on the oxidative conversion of 2',7'‑dichlorfluorescein‑diacetate (cell‑permeable) to dichlorofluorescein (fluorescent). The expression of p38MAPK was determined by western blotting. The expression level of p‑p38MAPK in H9c2 cells, which was significantly increased by exposure to 5 µM DOX for 60 min (P<0.01), was significantly decreased by pretreatment with 1 µM NRG for 150 min beforehand (P<0.01). The viability of H9c2 cells pretreated for 150 min with 1 µM NRG was significantly enhanced compared with that using DOX directly (P<0.01). Intracellular ROS levels were significantly reduced by being pretreated with 1 µM NRG for 150 min or with 3 µM SB203580 for 60 min before the cells were exposed to 5 µM DOX. Collectively, NRG protected H9c2 cells against the cardiotoxicity induced by DOX through suppressing the expression and activity of the p38MAPK pathway. The findings provided valuable evidence for the possible use of NRG to relieve DOX‑induced cardiotoxicity.

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