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Apelin‑13 promotes cell proliferation in the H9c2 cardiomyoblast cell line by triggering extracellular signal‑regulated kinase 1/2 and protein kinase B phosphorylation

  • Authors:
    • Luhua Yin
    • Pu Zhang
    • Chao Li
    • Jiafeng Si
    • Yongmei Wang
    • Xuemei Zhang
    • Deqing Zhang
    • Huanyi Zhang
    • Cong Lin

  • View Affiliations

  • Published online on: October 27, 2017     https://doi.org/10.3892/mmr.2017.7919
  • Pages: 447-451
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Apelin‑13 (APL‑13), a peptide hormone that serves as a ligand for G‑protein coupled receptors, has been demonstrated to be highly expressed in left ventricular hypertrophy rat models. It has been implicated in cardio‑protection under pathological states. The present study aimed to assess the physiological proliferation effect of APL‑13 in cultured H9c2 cardiomyoblast cells, and to elucidate the underlying mechanisms. Cell proliferation was determined by MTT assay. The extracellular signal‑regulated kinase (ERK) 1/2 and protein kinase B (Akt) signaling pathway was identified, and protein expression levels were detected using western blot analysis. The results demonstrated that APL‑13 markedly increased cell proliferation. Western blotting results suggested that APL‑13 significantly enhanced the expression of phosphoinositide ERK1/2 and Akt activation in a dose‑dependent manner. U0126 (10 µM; ERK1/2 inhibitor) and/or 10 µM LY294002 (Akt inhibitor) were used to help to determine the APL‑signaling mechanism. As a result, LY294002 and U0126 partially blocked the APL‑13 induced H9c2 proliferation. In conclusion, these data suggested that APL‑13 has a proliferative effect on myocardium cells via the Akt and ERK1/2 signaling pathways, and provide potential novel pharmaceutical targets for cardiovascular disease.
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January-2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
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  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Yin L, Zhang P, Li C, Si J, Wang Y, Zhang X, Zhang D, Zhang H and Lin C: Apelin‑13 promotes cell proliferation in the H9c2 cardiomyoblast cell line by triggering extracellular signal‑regulated kinase 1/2 and protein kinase B phosphorylation. Mol Med Rep 17: 447-451, 2018.
APA
Yin, L., Zhang, P., Li, C., Si, J., Wang, Y., Zhang, X. ... Lin, C. (2018). Apelin‑13 promotes cell proliferation in the H9c2 cardiomyoblast cell line by triggering extracellular signal‑regulated kinase 1/2 and protein kinase B phosphorylation. Molecular Medicine Reports, 17, 447-451. https://doi.org/10.3892/mmr.2017.7919
MLA
Yin, L., Zhang, P., Li, C., Si, J., Wang, Y., Zhang, X., Zhang, D., Zhang, H., Lin, C."Apelin‑13 promotes cell proliferation in the H9c2 cardiomyoblast cell line by triggering extracellular signal‑regulated kinase 1/2 and protein kinase B phosphorylation". Molecular Medicine Reports 17.1 (2018): 447-451.
Chicago
Yin, L., Zhang, P., Li, C., Si, J., Wang, Y., Zhang, X., Zhang, D., Zhang, H., Lin, C."Apelin‑13 promotes cell proliferation in the H9c2 cardiomyoblast cell line by triggering extracellular signal‑regulated kinase 1/2 and protein kinase B phosphorylation". Molecular Medicine Reports 17, no. 1 (2018): 447-451. https://doi.org/10.3892/mmr.2017.7919