Schisandrin B protects against myocardial ischemia/reperfusion injury via the PI3K/Akt pathway in rats

Zhao,Xuyong; Xiang,Yijia; Cai,Changhong; Zhou,Aiming; Zhu,Ning; Zeng,Chunlai

doi:10.3892/mmr.2017.7926

Schisandrin B protects against myocardial ischemia/reperfusion injury via the PI3K/Akt pathway in rats

Authors:
- Xuyong Zhao
- Yijia Xiang
- Changhong Cai
- Aiming Zhou
- Ning Zhu
- Chunlai Zeng
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Affiliations: Department of Cardiology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui Central Hospital, Lishui, Zhejiang 323000, P.R. China
Published online on: October 27, 2017 https://doi.org/10.3892/mmr.2017.7926
Pages: 556-561

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Abstract

The natural medicinal monomer, schisandrin B (Sch B), has been shown to exert cardioprotective effects; however, the underlying mechanisms of these effects remain to be fully elucidated. Therefore, the aim of the present study was to investigate whether Sch B attenuated myocardial ischemia/reperfusion (I/R) injury via the phosphoinositide 3‑kinases (PI3K)/Akt signaling pathway. To confirm this, I/R models were established in rats by ligation of the left anterior descending coronary artery. A group of animals were administered with Sch B (60 mg/kg, lavage) and/or the PI3K inhibitor, LY294002 (0.3 mg/kg, intraperitoneal). Myocardial infarct size, myocardial infarct serum markers, myocardial apoptotic index and the expression of Akt were measured in each group. The results demonstrated that the administration of Sch B reduced the size of the myocardial infarct, and this effect was eliminated following LY294002 treatment. In addition, the administration of Sch B decreased the apoptotic index and the serum markers of myocardial infarction. Sch B administration also increased the expression of phosphorylated Akt, and Sch B treatment decreased the B‑cell lymphoma 2 (Bcl‑2)‑like protein 4/Bcl‑2 ratio and the expression of cleaved caspase‑3. Therefore, Sch B may protect myocardial tissue from I/R injury via the PI3K/Akt signaling pathway in rats.

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