Comparison of the osteogenic differentiation of orofacial bone marrow stromal cells prior to and following marsupialization in patients with odontogenic cyst

Sun,Yao; Zhang,Juan; Qian,Naiying; Sima,Guoqi; Zhang,Jianming; Zhong,Jiayong; Guo,Zhiqin; Chen,Yawen; Dong,Weijie

doi:10.3892/mmr.2017.7949

Comparison of the osteogenic differentiation of orofacial bone marrow stromal cells prior to and following marsupialization in patients with odontogenic cyst

Authors:
- Yao Sun
- Juan Zhang
- Naiying Qian
- Guoqi Sima
- Jianming Zhang
- Jiayong Zhong
- Zhiqin Guo
- Yawen Chen
- Weijie Dong
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Affiliations: Department of Stomatology, The First Hospital of Jiaxing, Jiaxing, Zhejiang 314000, P.R. China, Department of Stomatology, Zhenjiang Stomatological Hospital, Zhenjiang, Jiangsu 212000, P.R. China
Published online on: November 3, 2017 https://doi.org/10.3892/mmr.2017.7949
Pages: 988-994
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Decompression has been considered a valuable tool for odontogenic cystic lesions to minimize cyst size with low morbidity and recurrence. However, whether decompression has a role in regulating stem cell properties of orofacial bone marrow stromal cells (BMSCs) around the cysts has not been fully investigated. The present study compared the stem cell marker profile and osteogenic differentiation potential of orofacial BMSCs prior to and following marsupialization (pre‑BMSCs vs. post‑BMSCs) in the same individuals. The results demonstrated that post‑BMSCs proliferated significantly faster, displayed higher colony‑forming unit‑fibroblast capacity and demonstrated higher expression of octamer binding protein 4, Nanog and SRY‑related HMG box 2 when compared with the pre‑BMSCs. Notably, the osteogenic potential was greater in the post‑BMSCs compared with in pre‑BMSCs, by demonstrating that the protein and mRNA expression levels of osteopontin, runt‑related transcription factor 2, osteocalcin, alkaline phosphatase and osterix were upregulated in pre‑BMSCs. Furthermore, the phosphorylated levels of extracellular signal‑regulated kinase and c‑Jun N‑terminal kinase were enhanced in post‑BMSCs. In conclusion, the study indicated that decompression influences the stem cell properties of orofacial BMSCs, and further studies are needed to verify the findings.

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