Open Access

Downregulation of TRIM28 inhibits growth and increases apoptosis of nude mice with non‑small cell lung cancer xenografts

  • Authors:
    • Lei Liu
    • Lei Zhang
    • Jianping Wang
    • Xuerong Zhao
    • Qian Xu
    • Yanjie Lu
    • Yanzhen Zuo
    • Long Chen
    • Jia Du
    • Yali Lian
    • Qin Zhang
  • View Affiliations

  • Published online on: November 3, 2017     https://doi.org/10.3892/mmr.2017.7955
  • Pages: 835-842
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

TRIM28 is a well‑known transcriptional co‑repressor of Kruppel‑associated box zinc finger proteins. The authors previously demonstrated that TRIM28 small interfering (si)RNA decreases cell proliferation and inhibits cell cycle progression in non‑small cell lung cancer (NSCLC) cell lines. The present study further demonstrated that the stable silencing of TRIM28 expression by a specific siRNA lentivirus vector significantly inhibited the growth and exerted obvious anti‑tumor effects in nude mice. The results of the terminal deoxynucleotidyl transferase‑mediated deoxyuridine triphosphate nick‑end labeling assay indicated that TRIM28 knockdown increased apoptosis. Furthermore, TRIM28 knockdown decreased the expression of B cell lymphoma (Bcl)‑2 and increased the expression of Bcl‑2 associated X, apoptosis regulator and p53 at the gene and protein levels. Auto‑antibodies to TRIM28 were present in 12.32% of the sera of the patients with NSCLC. The results suggest that TRIM28 knockdown may be effective against NSCLC, and TRIM28 antibodies have the potential to act as novel diagnostic and therapeutic tools.
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January-2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu L, Zhang L, Wang J, Zhao X, Xu Q, Lu Y, Zuo Y, Chen L, Du J, Lian Y, Lian Y, et al: Downregulation of TRIM28 inhibits growth and increases apoptosis of nude mice with non‑small cell lung cancer xenografts. Mol Med Rep 17: 835-842, 2018.
APA
Liu, L., Zhang, L., Wang, J., Zhao, X., Xu, Q., Lu, Y. ... Zhang, Q. (2018). Downregulation of TRIM28 inhibits growth and increases apoptosis of nude mice with non‑small cell lung cancer xenografts. Molecular Medicine Reports, 17, 835-842. https://doi.org/10.3892/mmr.2017.7955
MLA
Liu, L., Zhang, L., Wang, J., Zhao, X., Xu, Q., Lu, Y., Zuo, Y., Chen, L., Du, J., Lian, Y., Zhang, Q."Downregulation of TRIM28 inhibits growth and increases apoptosis of nude mice with non‑small cell lung cancer xenografts". Molecular Medicine Reports 17.1 (2018): 835-842.
Chicago
Liu, L., Zhang, L., Wang, J., Zhao, X., Xu, Q., Lu, Y., Zuo, Y., Chen, L., Du, J., Lian, Y., Zhang, Q."Downregulation of TRIM28 inhibits growth and increases apoptosis of nude mice with non‑small cell lung cancer xenografts". Molecular Medicine Reports 17, no. 1 (2018): 835-842. https://doi.org/10.3892/mmr.2017.7955